Abstract
The epidemiological typing schemes based on serotyping, antibiotic susceptibility pattern, plasmid DNA profile, and protein patterns determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were evaluated for their usefulness in typing clinical isolates ofPseudomonas aeruginosa. The serotypability was lower (45%) than reported in the literature (85–95%). The most commonly found serotypes were O:1 (19%), O:11 (25%), O:6 (35%). The electrophoretic analysis of plasmid DNAs showed plasmids (molecular weight=1 to >40 megadaltons). Two plasmids of Mr 2 and 38 megadaltons were found in various serotypes. The restriction enzyme analysis of plasmid DNA showed identical DNA fragment patterns among distinct serotypes. The SDS-PAGE protein banding patterns of whole-cell proteins showed homogeneity among the strains. However, analysis of the soluble protein patterns of the strains showed sufficiently distinct protein profiles that can be used to differentiate between various strains. The results of this study demonstrate that the electrophoretic patterns of soluble proteins, in combination with plasmid DNA profile or serotyping, can be of value in the epidemiologic fingerprinting of clinical isolates ofPseudomonas aeruginosa.
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Hemming, V. G., Overall, J. C., Britt, M. R. Nosocomial infections in a newborn intensive care unit: results of forty-one months of surveillance. New England Journal of Medicine 1976, 294: 1310–1316.
Schaberg, D. R., Haley, R. W., Highsmith, A. K., Anderson, R. L., McGown, J. E. Nosocomial bacteriuria: a prospective study of case clustering and antimicrobial resistance. Annals of Internal Medicine 1980, 93: 420–424.
Papavassiliou, J., Papapetropoulou, M. Serotypes ofPseudomonas aeruginosa in clinical specimens in relation to antibiotic susceptibility. Journal of Clinical Microbiology 1982, 16: 458–463.
Mayo, M. S., Cook, W. L., Scheitzer, R. L., Ward, M. A. Antibiograms, serotypes and plasmid profiles ofPseudomonas aeruginosa associated with corneal ulcers and contact lens wear. Journal of Clinical Microbiology 1986, 24: 372–376.
Edmonds, P., Suskind, R. R., MacMillan, B. G., Holder, I. A. Epidemiology ofPseudomonas aeruginosa in burns hospital: evaluation of serological, bacteriophage and pyocin methods. Applied Microbiology 1972, 24: 213–218.
Brokopp, C. D., Gomez-Lus, R., Farmer, J. J. Serological typing ofPseudomonas aeruginosa: use of commercial antisera and live antigens. Journal of Clinical Microbiology 1977, 5: 640–649.
Farmer, J. J., III, Weinstein, R. A., Zierdt, C. H., Brokopp, C. D. Hospital outbreaks caused byPseudomonas aeruginosa: importance of serogroup O:11. Journal of Clinical Microbiology 1982, 16: 266–270.
Fyfe, J. A. M., Harris, G., Govan, J. R. W. Revised pyocin typing methods forPseudomonas aeruginosa. Journal of Clinical Microbiology 1984, 20: 47–50.
Lindberg, R. B., Latta, R. L. Phage typing ofPseudomonas aeruginosa: clinical and epidemiologic considerations. Journal of Infectious Diseases 1974, 130: 533–542.
Tompkins, L. S., Plorde, J. J., Falkow, S. Molecular analysis of R-factors from multiresistant nosocomial isolates. Journal of Infectious Diseases 1980, 141: 625–636.
Janda, J. M., Bottone, E. J. Pseudomonas aeruginosa enzyme profiling: predictor of potential invasiveness and use as an epidemiological tool. Journal of Clinical Microbiology 1981, 14: 55–60.
Cato, E. P., Hash, D. E., Holderman, L. V., Moore, W. E. C. Electrophoretic study ofClostridium species. Journal of Clinical Microbiology 1981, 14: 376–382.
Jackman, P. J. H., Pelczynska, S. Characterization ofCorynebacterium group JK by whole-cell protein patterns. Journal of General Microbiology 1986, 132: 1911–1915.
Swings, J., Kersters, K., DeLey, J. Numerical analysis of electrophoretic protein patterns ofZymomonas strains. Journal of General Microbiology 1976, 93: 266–271.
Hanna, J., Neil, S. D., O'Brien, J. J., Ellis, W. A. Comparison of aerotolerant and reference strains ofCampylobacter species by polyacrylamide gel electrophoresis. International Journal of Systemic Bacteriology 1983, 33: 143–146.
Tall, B. D., Nauman, R. K. Microscopic agglutination and polyacrylamide gel electrophoresis analyses of oral anaerobic spirochetes. Journal of Clinical Microbiology 1986, 24: 282–287.
Mocca, L. F., Frasch, C. E. Sodium-dodecyl sulfate-polyacrylamide gel typing system for characterization ofNeisseria meningitidis isolates. Journal of Clinical Microbiology 1982, 16: 240–244.
Washington, J. A., II Susceptibility tests: Agar dilution. In: E. H. Lennette, A. Balows, W. J. Hausler, H. J. Shadomy (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington, D.C., 1985, p. 967–971.
Crossa, J. H., Falkow, S. Isolation of plasmid DNA of large molecular weight. In: P. Gerhardt, R. G. E. Murray, R. N. Bostilow, E. W. Nester, W. A. Wood, N. R. Krieg, G. B. Phillips (ed.): Manual of methods for general bacteriology. American Society for Microbiology, Washington, D.C., 1985, p. 269–270.
Hansen, J. B., Olson, R. H. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids PMG1 and PMG5. Journal of Bacteriology 1978, 135: 227–238.
Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the protein-dye binding. Analytical Biochemistry 1976, 72: 248–254.
Moore, W. C. C., Hash, D. E., Holdeman, V., Cato, E. P. Polyacrylamide slab gel electrophoresis of soluble protein for studies on bacterial floras. Applied and Environmental Microbiology 1980, 39: 900–907.
Farmer, J. J. Mnemonic for reporting bacteriocin and bacteriophage types. Lancet 1976, ii: 96.
Walia, S. K., Duckworth, D. H. The relationship of the delta transfer factor, KColIBto the ColIb plasmid. Journal of General Microbiology 1986, 132: 3261–3268.
Hughes, V. M., Datta, N. Conjugative plasmids in bacteria of pre-antibiotic era. Nature 1983, 302: 725–726.
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Walia, S., Madhavan, T., Williamson, T. et al. Protein patterns, serotyping and plasmid DNA profiles in the epidemiologic fingerprinting ofPseudomonas aeruginosa . Eur. J. Clin. Microbiol. Infect. Dis. 7, 248–255 (1988). https://doi.org/10.1007/BF01963096
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DOI: https://doi.org/10.1007/BF01963096