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Characterization of purification-associated reduction in IgE-dependent histamine release from rat peritoneal mast cells

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Abstract

Histamine release from purified rat peritoneal mast cells (PMC) was examined and compared to that from a non-purified preparation (PEC). Both PEC and PMC released similar amounts of histamine upon stimulation with compound 48/80, calcium ionophore A23187 and substance P. In contrast, IgE-dependent histamine release from PMC caused by antigen, anti-IgE and concanavalin A was very low compared to that of PEC. The reduced IgE-dependent histamine release from PMC, however, was recovered when PMC was reconstituted with non-mast cells (NMC) present in the peritoneal cavity. The effect was time-dependent and reached a plateau in 30 min. NMC from both sensitized and non-sensitized rats recovered the reduced histamine release from PMC dose-dependently. The potentiating effect of NMC was observed even in the presence of excess amount of phosphatidylserine. Supernatants of NMC and a mixture of PMC and NMC incubated for 1 hr at 37°C, however, failed to potentiate the histamine release. These results demonstrate that IgE-dependent histamine release from rat peritoneal mast cells is upregulated by other cells present in the peritoneal cavity, and that the mechanism involved is distinct from that of phosphatidylserine.

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Authors and Affiliations

  1. Department of Pharmacology, Gifu Pharmaceutical University, 5-6-1 Mitahorahigashi, 502, Gifu, Japan

    N. Inagaki, H. Kawasaki & H. Nagai

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  1. N. Inagaki
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  2. H. Kawasaki
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  3. H. Nagai
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Inagaki, N., Kawasaki, H. & Nagai, H. Characterization of purification-associated reduction in IgE-dependent histamine release from rat peritoneal mast cells. Inflamm Res 44, 541–547 (1995). https://doi.org/10.1007/BF01757359

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  • DOI: https://doi.org/10.1007/BF01757359

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