Abstract
The clinical value of a new RNA-DNA hybridization assay for quantification of Cytomegalovirus (CMV) DNA in leukocytes [Hybrid Capture CMV DNA Assay (HCA); Murex Biotech, UK] was evaluated. The HCA was compared with an assay for CMV pp65 antigen in leukocytes and an in-house CMV polymerase chain reaction PCR (CMV-PCR) on parallel blood samples. The HCA and the CMV-PCR were less sensitive than the CMV pp65 assay, but the positive predictive value of all three methods for CMV disease was 50% or less. However, when quantitation of viral load by HCA and CMV pp65 assay was taken into consideration, both assays were superior to CMV-PCR in predicting CMV disease.
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Rollag, H., Sagedal, S., Holter, E. et al. Diagnosis of cytomegalovirus infection in kidney transplant recipients by a quantitative RNA-DNA hybrid capture assay for cytomegalovirus DNA in leukocytes. Eur. J. Clin. Microbiol. Infect. Dis. 17, 124–127 (1998). https://doi.org/10.1007/BF01682170
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DOI: https://doi.org/10.1007/BF01682170