Summary
A detailed immunohistochemical study has been carried out on 63 breast lesions with epitheliosis, ductal carcinoma in situ and clinging carcinoma (lobular cancerization), using antibodies directed against keratins 5/14 and 14, 15, 16, 18, 19, vimentin, smooth muscle actin, collagen IV and laminin. The results have shown that epitheliosis on the one hand and ductal in situ and clinging carcinoma on the other are immunohistochemically different epithelial lesions. Epitheliosis appears to be epithelial hyperplasia with keratin 5/14 and keratin 14, 15, 16, 18, 19-positive cells. Compared to epitheliotic cells tumor cells of clinging carcinoma, lobular cancerization and ductal carcinoma in situ expressed only luminal keratins 14, 15, 16, 18, 19 in 85% of the cases studied; whereas in 15% there was a basal keratin expression. From our results we conclude that the clinging carcinoma (lobular cancerization) represents the initial morphological step in the development of ductal carcinoma in situ and thus may be interpreted as a minimal ductal neoplasia. With the immunohistochemical demonstration of basal and luminal keratins it may be possible in individual cases to differentiate between benign and malignant in situ lesions of the breast.
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References
Azzopardi JG (1979) Problems in breast pathology. Saunders, London
Bässler R (1978) Pathologie der Brustdrüse. In: Doerr W, Seifert G, Uehlinger E (eds) Spezielle pathologie Anatomie. vol 2. Springer, Berlin Heidelberg New York
Black MM, Kwon S (1980) Precancerous mastopathy: structural and biological considerations. Pathol Res Pract 166:491–514
Böcker W, Jarasch ED, Nagle RB, Höhne R (1986) Morphology, topography, and patterns of benign epithelial proliferations and pre-invasive carcinoma. In: Bässler R, Hübner K (eds) Pathology of neoplastic and endocrine induced diseases of the breast. Gustav Fischer, Stuttgart, pp 72–78
Böcker W, Bier B, Freytag G, Brömmelkamp B, Jarasch ED, Edel G, Dockhorn-Dworniczak B (1992) An immunohistochemical study of the breast using antibodies to basal and luminal keratins, alpha-smooth muscle actin, vimentin, collagen IV and laminin. Part 1: normal breast and benign proliferative lesions. Virchows Arch [A] 421:315–322
Carter D (1990) Interpretation of breast biopsies. Raven Press, New York
Cordell JL, Falini B, Erber WN, Gosh AK, Abdulaziz Z, MacDonald S, Fulford KAF, Stein H, Mason DY (1984) Immuno enzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP) complex. J Histochem Cytochem 32:219–229
Dupont WD, Page DE (1985) Risk factors for breast cancer in women with proliferative breast disease. N Engl J Med 312:146–151
Eusebi V, Foschini MP, Cook MG, et al. (1989) Long-term follow-up of in situ carcinoma of the breast with special emphasis on clinging carcinoma. Semin Diagn Pathol 6:165–173
Fechner RE (1971) Ductal carcinoma involving the lobule of the breast. A source of confusion with lobular carcinoma in situ. Cancer 28:274–281
Fechner RE, Mills SE (1990) Breast pathology. Benign proliferations, atypias and in situ carcinomas. ASCP Press, Chicago
Ferenczy A, Winkler B (1987) Cervical intraepithelial neoplasia and condyloma. In: Kurman RJ (ed) Blaustein's pathology of the female genital tract. Springer, Berlin Heidelberg New York, pp 177–217
Gallager HS, Martin JE (1969) The study of mammary carcinoma by mammography and whole organ sectioning. Early observations. Cancer 23:855–873
Gould VE, Koukoulis GK, Jansson DS, Nagle RB, Franke WW, Moll R (1990) Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic and neoplastic breast. Am J Pathol 137:1143–1155
Gusterson BA, Monaghan P, Mahendran R, Ellis J, O'Hare MJ (1986) Identification of myoepithelial cells in human and rat breasts by anti-common acute lymphoblastic leukemia antigen antibody A12. J Natl Cancer Inst 77:343–349
Haagensen CD, Bodian C, Haagensen DE Jr (1981) Breast carcinoma. Risk and detection. Saunders, Philadelphia
Jarasch ED, Nagle RB, Kaufmann M, Maurer C, Böcker W (1988) Differential diagnosis of benign epithelial proliferations and carcinomas of the breast using antibodies to cytokeratins. Hum Pathol 19:276–289
Lagios MD, Margolin FR, Westdahl PR, Rose MR (1989) Mammographically detected duct carcinoma in situ. Frequeny of local recurrence following tylectomy and prognostic effect of nuclear grade on local recurrence. Cancer 63:618–624
Millis RR (1984) Atlas of breast pathology. In: Gresham GA (ed) Current histopathology, vol 7. MTP Press, Lancaster
Nagle RB, Böcker W, Davis JR, Heid HW, Kaufmann M, Lucas DO, Jarasch ED (1986) Characterization of breast carcinomas by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells. J Histochem Cytochem 34:869–881
Ober KG, Tulusan AG (1985) Tumoren der Brustdrüse. In: Gross R, Schmidt CG (eds) Klinische Onkologie. Thieme, Stuttgart
Page DL (1988) Anatomic markers of human mammary premalignancy and incipient breast cancer. Rec Results Cancer Res 106:65–72
Page DL, Anderson TJ (1987) Diagnostic histopathology of the breast. Churchill-Livingstone, Edinburgh
Page DL, Van Der Zwaag R, Rogers LW, Williams LT, Walter WE, Hartmann WH (1978) Relation between component parts of fibrocystic disease complex and breast cancer. J Natl Cancer Inst 61:1055–1063
Potten CS (1979) Stem cells and tissue homeostasis. In: Lord BJ, Potten CS, Cole RJ (eds) Stem cells. Cambridge University Press, Cambridge
Prechtel K (1972) Beziehungen der Mastopathie zum Mammakarzinom. Fortschr Med 90:41–80
Prechtel K (1974) Allgemeine Erläuterungen zur Histomorphologie von Brustdrüsenerkrankungen. Fortschr Med 92:374–380
Purkis RE, Steel JW, MacKenzie JC, Nathrath WB, Leigh IM, Lane EB (1990) Antibody markers of basal cells in complex epithelia. J Cell Science 97:39–50
Raju U, Crissman JD, Zarbo RJ, Gottlieb C (1990) Epitheliosis of the breast. Am J Surg Pathol 14:939–947
Rosai D (1991) Borderline epithelial lesions of the breast. Am J Surg Pathol 15:209–221
Russo J, Russo IH (1980) Influence of differentiation and cell kinetics on the susceptibility of the rat mammary gland to carcinogenesis. Cancer Res 40:2677–2687
Stegner HE (1975) Pathologisch-anatomische Aspekte der organerhaltenden (konservierenden) Therapie bei Karzinom-Früh-stadien der Mamma. Oesterr Z Onkol 2:136–144
Viale G, Gambacorta M, Coggi G, Dell'Orto P, Milani M, Doggnlioni C (1991) Glial fibrillary acidic protein immunoreactivity in normal and diseased human breast. Virchows Arch [A] 418:339–348
Wellings SR, Jensen MM, Marcum RG (1975) An atlas of subgross pathology. J Natl Cancer Inst 55:231–273
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Dedicated to Prof. Dr. E. Grundmann on the occasion of his 70th birthday
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Böcker, W., Bier, B., Freytag, G. et al. An immunohistochemical study of the breast using antibodies to basal and luminal keratins, alpha-smooth muscle actin, vimentin, collagen IV and laminin. Vichows Archiv A Pathol Anat 421, 323–330 (1992). https://doi.org/10.1007/BF01660979
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DOI: https://doi.org/10.1007/BF01660979