Abstract
Four anti-HLA-A3 sera crossreactive with HLA-A11 were tested with a panel of A3 positive cells pretreated with either Fab'2 prepared from one of the four anti-A3 sera or Fab'2 fragments of an anti-A11 serum displaying CYNAP crossreactivity with A3. The anti-A3 Fab'2 blocked the reactions of all anti-A3 sera with all A3 +, 11− cells; the anti-A11 Fab'2 blocked the reactions of only some serum-cell combinations. Each of the four anti-A3 sera tested were blocked by the anti-A11 Fab'2 in a different pattern, indicating that each could have a different anti-A3 specificity. Five different patterns of reactivity were observed among the 21 test lymphocytes. These findings are interpreted as evidence for shared antigenic components between A3 and A11.
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Reisner, E.G., Legrand, L. & Dausset, J. Specificities shared between HLA-A3 and HLA-A11 detected by Fab'2 blocking. Immunogenetics 3, 233–240 (1976). https://doi.org/10.1007/BF01576956
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DOI: https://doi.org/10.1007/BF01576956