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Evidence for structural homology between murine and human Ia antigens

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Abstract

The serological cross-reactivity and the structural homology of murine and human Ia alloantigens were analyzed. Both normal human peripheral blood B lymphocytes and chronic lymphocytic leukemia (CLL) cells were shown to be lysed in the presence of complement by both murine anti-Ia and human anti-HLA-DR alloantisera. A mouse A.TH anti-A.TL (anti-I k) alloantiserum reacted with determinants expressed on all of the 20 normal human B cell populations tested. Only 3 of these 20 B cell populations were lysed with an A.TL anti-A.TH anti-I s alloantiserum. The frequency of cytotoxic cross-reactivity concordant with anti-I k appears to be greater for anti-I-EC k than for anti-I-A k alloreactivity.

An immunochemical analysis demonstrated that Iaα-chain andβ-chain polypeptides may be immunoprecipitated from CLL cell lysates by either a mouse anti-I k alloantiserum or various human anti-HLA-DR alloantisera. The Ia molecules detected with the mouse and human antisera are coprecipitable as revealed by one-dimensional gel electrophoresis. Two-dimensional gel electrophoresis studies indicated that the human CLL cell Ia antigens analyzed possess considerable molecular heterogeneity. They are structurally more similar, with respect to molecular size and charge, to mouse Ia antigens determined by the murineH-2-linkedI-EC subregion rather than theI-A subregion. The structural, genetic and functional implications of these findings are discussed.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research, C. H. Best Institute, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada

    Terry L. Delovitch

  2. Departments of Pathology and Surgery, University of Toronto, M5G 1L6, Toronto, Canada

    Judy A. Falk

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  1. Terry L. Delovitch
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  2. Judy A. Falk
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Additional information

As suggested by the nomenclature committee at theIr Gene Workshop in Asilomar, December 1976, Ia polypeptides are designated by a capital letter denoting the appropriate subregion with the haplotype designated by a superscript and the subunit component by a subscript. Since it has proven difficult to serologically and immunologically resolveI-E andI-C subregion products, this chromosome segment is tentatively designated asI-EC.

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Delovitch, T.L., Falk, J.A. Evidence for structural homology between murine and human Ia antigens. Immunogenetics 8, 405–418 (1979). https://doi.org/10.1007/BF01561452

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