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Effect of focal cerebral ischemia on nitric oxide synthase expression in rats

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Abstract

Time- and cell-type-dependent immunohistochemical activity of nitric oxide synthase (NOS) was investigated in rat cerebral cortex following focal ischemia and the local concentration of nitric oxide (NO) was measured. NO concentration increased 2 min after the ischemia. Brain NOS-immunoreactive neurons increased in number 5 min after the ischemia. Endothelial cell NOS immunoreactivity was first detected in vascular endothelial cells and astrocytes 5 min after the ischemia, and it increased again during 60 min to 4 days after the ischemia in reactive astrocytes. Inducible NOS immunoreactivity was detected in astrocytes, vascular endothelium, and microglia/macrophages at the periphery of the ischemic core during 2–4 days after the ischemia.

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Authors and Affiliations

  1. Department of Neurosurgery, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, 142, Tokyo, Japan

    Hideki Homma, Hidekatsu Mizushima, Yoshinori Arai, Kenji Dohi & Kiyoshi Matsumoto

  2. Department of Anatomy, Showa University School of Medicine, Tokyo, Japan

    Seiji Shioda & Yasumitsu Nakai

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  1. Hideki Homma
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  2. Hidekatsu Mizushima
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  3. Yoshinori Arai
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  4. Kenji Dohi
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  5. Kiyoshi Matsumoto
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  6. Seiji Shioda
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  7. Yasumitsu Nakai
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Homma, H., Mizushima, H., Arai, Y. et al. Effect of focal cerebral ischemia on nitric oxide synthase expression in rats. Med Electron Microsc 30, 55–62 (1997). https://doi.org/10.1007/BF01545082

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  • DOI: https://doi.org/10.1007/BF01545082

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