Summary
In 1983, a parvo-like virus (Yamanashi isolate) was newly isolated from silkworm. However, unlike parvovirus, two DNA molecules (VD1 and 2) were always extracted from purified virions. To investigate the structure and organization of the virus genomes, we determined the complete nucleotide sequence of VD2. The sequence consisted of 6031 nucleotides (nts) and contained a large open reading frame (ORF1) with 3513 nts. A smaller open reading frame (ORF2) with 702 nts was found in the complementary sequence. Computer analysis revealed that both ORFs did not code for the major structural proteins (VP1, 2, 3, and 4). These results suggest that VD2 has not enough information to produce progeny virions by itself. Further, the structural importance of the terminal sequence (CTS) common to both VD1 and VD2 was also predicted by a computer analysis.
References
Alberts B, Bray D, Lewis J, Raff M, Roberts K, Waston JD (1989) Molecular biology of the cell, 2nd ed. Garland, New York, pp 551–612
Bando H, Choi H, Ito Y, Kawase S (1990) Terminal structure of a densovirus implies a hairpin transfer replication which is similar to the model for AAV. Virology 179: 57–63
Bando H, Choi H, Ito Y, Nakagaki M, Kawase S (1992) Structural analysis on the single-stranded genomic DNAs of the virus newly isolated from silkworm: the DNA molecules share a common terminal sequence. Arch Virol 124: 187–193
Berg J (1988) Proposed structure for the zinc-binding domains from transcription factor IIIA and related proteins. Proc Natl Acad Sci USA 85: 99–102
Blissard GW, Rohrman GF (1990) Baculovirus diversity and molecular biology. Annu Rev Entomol 35: 127–155
Cavener DR (1987) Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates. Nucleic Acids Res 15: 1353–1361
DePamphilis ML (1988) Transcriptional elements as comsponents of eukaryotic origins of DNA replication. Cell 52: 635–638
Gidoni D, Dynan WS, Tjian R (1994) Multiple specific contacts between a mammalian transcription factor and its congnate promoters. Nature 312: 409–413
Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–369
Ikemura T (1982) Correlation between the abundance of yeast transfer RNAs and occurrence of the respective codons in protein genes. J Mol Biol 158: 573–597
Kawase S, Cai You-min, Bando H, Seki H (1984) Chemical properties of Yamanashi isolate of theBombyx densonucleosis virus. J Ser Sci Jpn 53: 341–347
Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosome. Cell 44: 283–292
Kurihara Y, Watanabe H, Maeda S, Shimizu T (1984) Chemical characteristics of a previously undescribed densonucleosis virus isolated from theSilkworm, Bombyx mori. J Ser Sci Jpn 53: 33–40
McKnight SL, Kingsbury R (1982) Transcriptional control signals of a eukaryotic protein-coding genes. Science 217: 316–324
Nakagaki M, Kawase S (1980) DNA of a new parvo-like virus isolated from the silkworm,Bombyx mori. J Invertebr Pathol 35: 124–133
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Seki H, Iwashita Y (1983) Histopathological features and pathogenicity of a densonucleosis virus of the silkworm,Bombyx mori, isolated from sericultural farms in Yamanashi prefecture. J Seric Sci Jpn 52: 400–405
Siegle G, Bates RC, Berns KI, Carter BJ, Kelly DC, Kurstak E, Tattersall P (1985) Characteristics and taxonomy of parvoviridae. Intervirology 23: 61–73
Suzuki T, Suzuki Y (1988) Interaction of composite protein complex with the fibroin enhancer sequence. J Biol Chem 263: 5979–5986
Tijssen P, Arella M (1991)Parvoviridae. Structure and replication of densonucleosis virus. In Adams JR, Bonami R (eds), Atlas of invertebrate viruses. CRC Press, Boca Raton, pp 41–54
Watanabe H, Kawase S, Shimizu T, Seki H (1986) Differences of serological characteristics of densonucleosis viruses in the silkworm,Bombyx mori. J Ser Sci Jpn 55: 75–76
Zuker M, Stiegler P (1981) Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Res 9: 133–148
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bando, H., Hayakawa, T., Asano, S. et al. Analysis of the genetic information of a DNA segment of a new virus from silkworm. Archives of Virology 140, 1147–1155 (1995). https://doi.org/10.1007/BF01315423
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01315423