Summary
We studied the competitive growth among SA11-L2(G3) and its single-human VP7 gene-substitution reassortants SA11-L2/KU-R1(G1) and SA11-L2/DS1-R1(G2), which have the genetic background of SA11-L2, during sequential passages after mixed infection. When the same infectious units (m.o.i. of 5 p.f.u./cell) of SA11-L2 and a reassortant SA11-L2/KU-R1 were inoculated onto and passaged in MA104 cells, 88% of the virus clones isolated from the culture fluid at the 3rd passage belonged to G3, and all the clones from the 10th passage had G3 specificity. Even when SA11-L2/KU-R1 with titer 10 times higher than that of SA11-L2 was used in the coinfection, the predominance of clones with G3-VP7 was observed. Although G2 clones slightly surpassed G1 clones in number in the mixed culture of SA11-L2/KU-R1 and SA11-L2/DS1-R1, G3 clones predominated in the virus progeny from a mixed culture infected with the same titers of SA11-L2, SA11-L2/KU-R1, and SA11-L2/DS1-R1. However, no significant difference in viral growth was detected among SA11-L2 and the two reassortants.
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References
Burns JW, Chen D, Estes MK, Ramig RF (1989) Biological and immunological characterization of a simian rotavirus SA11 variant with an altered genome segment 4. Virology 169: 427–435
Chen D, Estes MK, Ramig RF (1992) Specific interactions between rotavirus outer capsid proteins VP4 and VP7 determine expression of a cross-reactive, neutralizing VP4-specific epitope. J Virol 66: 432–439
Clark HF, Borian FE, Modesto K, Plotkin SA (1990) Serotype 1 reassortant of bovine rotavirus WC3, strain WI79-9, induces a polytypic antibody response in infants. Vaccine 8: 327–332
Dagan R, Kassis I, Sarov B, Midthun K, Davidson BL, Vesikari T, Sarov I (1992) Safety and immunogenicity of oral tetravalent human-rhesus reassortant rotavirus vaccine in neonates. Pediatr Infect Dis J 11: 991–996
Flores J, Perez-Schael I, Blanco M, White L, Garcia D, Vilar M, Cunto W, Gonzalez R, Urbina C, Boher J, Mendez M, Kapikian AZ (1990) Comparison of reactogenicity and antigenicity of M37 rotavirus vaccine and rhesus-rotavirus-based quadrivalent vaccine. Lancet 336: 330–334
Flores J, Kapikian AZ (1992) Rotavirus vaccines. In: Ellis RW (ed) Vaccines: new approaches to immunological problems. Butterworth-Heinemann, Stoneham, pp 255–288
Gorziglia M, Larralde G, Kapikian AZ, Chanock RM (1990) Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci USA 87: 7155–7159
Halsey NA, Anderson EL, Sears SD, Steinhoff M, Wilson M, Belshe RB, Midthun K, Kapikian AZ, Chanock RM, Samorodin R, Burns B, Clements ML (1988) Humanrhesus reassortant rotavirus vaccines: safety and immunogenicity in adults, infants, and children. J Infect Dis 158: 1261–1267
Kobayashi N, Kojima K, Taniguchi K, Urasawa T, Urasawa S (1994) Genotypic diversity of reassortants between simian rotavirus SA11 and human rotaviruses having different antigenic specificities and RNA patterns. Res Virol 145: 303–311
Kool DA, Matsui SM, Greenberg HB, Holmes IH (1992) Isolation and characterization of a novel reassortant between avian Ty-1 and simian RRV rotaviruses. J Virol 66: 6836–6839
Midthun K, Greenberg HB, Hoshino Y, Kapikian AZ, Wyatt RG, Chanock RM (1985) Reassortant rotaviruses as potential live rotavirus vaccine candidates. J Virol 53: 949–954
Midthun K, Hoshino Y, Kapikian AZ, Chanock RM (1986) Single gene substitution rotavirus reassortants containing the major neutralization protein (VP7) of human rotavirus serotype 4. J Clin Microbiol 24: 822–826
Ni Y, Kemp MC (1992) Strain-specific selection of genome segments in avian reovirus coinfections. J Gen Virol 73: 3107–3113
Perez-Schael I, Blanco M, Vilar M, Garcia D, White L, Gonzalez R, Kapikian AZ, Flores J (1990) Clinical studies of a quadrivalent rotavirus vaccine in Venezuelan infants. J Clin Microbiol 28: 553–558
Steele AD, Garcia D, Sears J, Gerna G, Nakagomi O, Flores J (1993) Distribution of VP4 gene alleles in human rotaviruses by using probes to the hyperdivergent region of the VP4 gene. J Clin Microbiol 31: 1735–1740
Taniguchi K, Urasawa T, Morita Y, Greenberg HB, Urasawa S (1987) Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis 155: 1159–1166
Taniguchi K, Nishikawa K, Kobayashi N, Urasawa T, Wu H, Gorziglia M, Urasawa S (1994) Differences in plaque size and VP4 sequence found in SA11 virus clones having simian authentic VP4. Virology 198: 325–330
Ward RL, Knowlton DR, Hurst P-F (1988) Reassortant formation and selection following coinfection of cultured cells with subgroup 2 human rotaviruses. J Gen Virol 69: 149–162
Xu Z, Woode GN (1993) Studies on the role of VP4 of G serotype 10 rotavirus (B223) in the induction of the heterologous immune response in calves. Virology 196: 294–297
Xu Z, Woode GN (1994) Studies on the influence of the VP7 gene on rotavirus replication. Virology 198: 394–398
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Kobayashi, N., Taniguchi, K., Kojima, K. et al. Preferential selection of VP7 gene from a parent rotavirus strain (SA11) in sequential passages after mixed infection with SA11 and SA11-human rotavirus single-VP7 gene-substitution reassortants. Archives of Virology 140, 775–781 (1995). https://doi.org/10.1007/BF01309965
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DOI: https://doi.org/10.1007/BF01309965