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Haemoglobin synthesis and cell wall regeneration by electric field-induced interkingdom heterokaryons

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Summary

Animal-plant heterokaryons were produced by electrically-induced fusion between mouse erythroleukaemic cells (Friend cells) andPetunia protoplasts. Such heterokaryons exhibited the animalspecific function of DMSO-stimulated haemoglobin synthesis, and the plant-specific function of cell wall regeneration.

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Abbreviations

DMSO:

dimethylsulfoxide

2,4-D:

2,4-dichlorophenoxyacetic acid

IDAA:

iminodiacetic acid

6-BAP:

6-benzyl amino purine

NAA:

1-naphthalene acetic acid

MS:

Murashige and Skoog Medium

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Salhani, N., Vienken, J., Zimmermann, U. et al. Haemoglobin synthesis and cell wall regeneration by electric field-induced interkingdom heterokaryons. Protoplasma 126, 30–35 (1985). https://doi.org/10.1007/BF01287670

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