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Action du bromhydrate d'éthidium sur le développement du virus vaccinal dans des cellules en culture de rein de babouin

Action of Ethidium Bromide on the development of vaccinia virus cultured in baboon kidney cells

I. Action sur la cinétique de développement viral

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Résumé

Le bromhydrate d'éthidium (BET) inactive à 37°C le virus de la vaccine. Appliqué à partir de la 3ème ou de la 6ème heure après inoculation à des cellules de rein de babouin infectées par ce virus, il inhibe presque complètement la formation de virions; appliqué à partir de la 12ème heure il inhibe partiellement le développement viral, il est sans effet sur celui-ci à partir de la 18ème heure. Le prétraitement des cellules diminue, s'il est pratiqué dans les 24 heures précédant l'inoculation, le rendement en virions. La complexation par le BET des ADN cellulaire et viral pourrait être à l'origine de l'action inhibitrice de ce produit.

Summary

Ethidium Bromide (EB) inactivates the vaccinia virus at 37°C. It almost completely inhibits the formation of virions when applied at the 3rd or the 6th hour following infection of baboon kidney cells by this virus. Viral development is partially inhibited when EB is applied after the 12th hour and not affected after the 18th hour. Pre-treatment of the cells within the 24 hours previous to infection lowers the yield of virions. The complexation by EB of cellular and viral DNA could be the cause of the inhibitory action of this product.

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  1. R. Vilaginès

    Present address: Dr. McAuslan's Laboratory, Roche Institute of Molecular Biology, 07110, Nutley, NJ, USA

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  1. Service des Virus, Institut Pasteur, Paris, France

    R. Vilaginès

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Vilaginès, R. Action du bromhydrate d'éthidium sur le développement du virus vaccinal dans des cellules en culture de rein de babouin. Archiv f Virusforschung 30, 59–66 (1970). https://doi.org/10.1007/BF01262583

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  • DOI: https://doi.org/10.1007/BF01262583

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