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On the mechanism of the yolk-lysis reaction

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Summary

  1. (1)

    The reaction by which yolk platelets isolated from frog eggs undergo lysis in the presence of low concentrations of calcium ions is described and kinetic data are presented.

  2. (2)

    A crude yolk precipitate, prepared by salting-in and reprecipitating the material of intact yolk platelets, undergoes the same reaction as do the intact platelets.

  3. (3)

    Data are presented on the pH dependence and thermal inhibition of the reaction.

  4. (4)

    The yolk contains a material, perhaps a protein, which catalyzes the lysis reaction. This material is not the soluble product of the reaction, since the kinetics are not those of an autocatalytic process.

  5. (5)

    A possible mechanism for the reaction is discussed.

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Literature cited

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Authors and Affiliations

  1. Zoological Laboratory, University of Pennsylvania, Philadelphia, Pennsylvania

    Paul R. Gross

Authors
  1. Paul R. Gross
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Additional information

This investigation was supported by a research grant from the National Cancer Institute, National Institutes of Health, Public Health service, administered by L. V. Heilbrunn.

National Science Foundation predoctoral research fellow.

The author wishes to thank Dr. L. V. Heilbrunn for his helpful suggestions and criticism.

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Gross, P.R. On the mechanism of the yolk-lysis reaction. Protoplasma 43, 416–428 (1954). https://doi.org/10.1007/BF01248279

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  • DOI: https://doi.org/10.1007/BF01248279

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