Skip to main content
SpringerLink
Log in

Direct electron-microscopy of organ cultures for the detection and characterization of viruses

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

Organ cultures were inoculated with viruses belonging to five different morphological groups. Cells and debris were abraded from the cultures four days later and examined by a negative contrast technique. All but the smallest particles were readily recognised. This method has been of value in visualising some recently isolated viruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hoorn, B.: Organ cultures of ciliated epithelium for the study of respiratory viruses. Acta path. microbiol. scand.66, Supp. 183 (1966).

    Google Scholar 

  2. Tyrrell, D. A. J., andM. L. Bynoe: Cultivation of a novel type of common cold virus in organ cultures. Brit med. J.1, 1467–1470 (1965).

    Google Scholar 

  3. Hoorn, B., andD. A. J. Tyrrell: A new virus cultivated only in organ cultures of human ciliated epithelium. Arch. ges. Virusforsch.18, 210–225 (1966).

    Google Scholar 

  4. Lwoff, A., R. Horne, andP. Tournier: A system of viruses. Cold Spr. Harb. Symp. quant. Biol.27, 51–55 (1962).

    Google Scholar 

  5. Hamparian, V. V., M. R. Hilleman, andA. Ketler: Contributions to characterization and classification of animal viruses. Proc. Soc. exp. Biol. (N.Y.)112, 1040–1050(1963).

    Google Scholar 

  6. Waterson, A. P., andJ. D. Almeida: Taxonomic implications of “Myxovirus”. Nature (Lond.)210, 1138–1140 (1966).

    Google Scholar 

  7. Home, R. E., andP. Wildy: Symmetry in virus architecture. Virology15, 348–373 (1961).

    Google Scholar 

  8. Almeida, J. D., andD. A. J. Tyrrell: J. gen. Virol.1 (1967).

  9. Almeida, J. D.: A classification of virus particles based, on morphology. Canad. med. Ass. J.89, 787–798 (1963).

    Google Scholar 

  10. Hamre, D., andJ. J. Procknow: A new virus isolated from the human respiratory tract. Proc. Soc. exp. Biol. (N. Y.)121, 190–193 (1966).

    Google Scholar 

  11. Berry, D. M., J. Cruickshank, H. P. Chu, andR. J. H. Wells: The structure of infectious bronchitis virus. Virology23, 403–407 (1964).

    Google Scholar 

  12. Mallucci, L., andJ. A. Almeida: Unpublished observation.

Download references

Author information

Author notes

  1. D. A. J. Tyrrell

    Present address: Medical Research Council Common Cold Research Unit, Salisbury, Wilts

Authors and Affiliations

  1. Medical Research Council Common Cold Research Unit, Salisbury, Wilts

    June D. Almeida

  2. Departments of Medical Microbiology, St. Thomas' Hospital Medical School, London, S. E. 1., England

    D. A. J. Tyrrell & June D. Almeida

Authors
  1. D. A. J. Tyrrell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. June D. Almeida
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tyrrell, D.A.J., Almeida, J.D. Direct electron-microscopy of organ cultures for the detection and characterization of viruses. Archiv f Virusforschung 22, 417–425 (1967). https://doi.org/10.1007/BF01242962

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01242962

Keywords

Search

Navigation