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Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine uromodulin (Tamm-Horsfall glycoprotein)

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Abstract

The urinary glycoprotein uromodulin (Tamm-Horsfall glycoprotein) exhibits a pregnancy-associated ability to inhibit antigen-specific T cell proliferation, and the activity is associated with a carbohydrate moiety [Muchmore and Decker (1985) Science 229:479–81; Hessionet al., (1987) Science 237:1479–84; Muchmore, Shifrin and Decker (1987) J Immunol 138:2547–53]. We report here that the Man6(7)GlcNAc2-R glycopeptides derived from uromodulin inhibit antigen-specific T cell proliferation by 50% at 0.2–2 μM, and further studies, reported elsewhere, confirm that oligomannose glycopeptides from other sources are also inhibitory, with Man9GlcNAc2-R the most inhibitory of those tested [Muchmoreet al., J Leukocyte Biol (in press)]. In this work, we have extended the observation of pregnancy-associated inhibitory activity to a second species, and have compared the oligomannose profile of Tamm-Horsfall glycoprotein (nonpregnant) with that of uromodulin (pregnant) derived from both human and bovine sources. Surprisingly, there was a pregnancy-associated decrease in the total content of oligomannose chains due predominantly to a reduction in Man5GlcNAc2-R and Man6GlcNAc2-R. Man7GlcNAc2-R, which did not decrease with pregnancy, comprised a significantly greater proportion of the total oligomannose chains in pregnant vs. nonpregnant samples from both species (human; 34.6% vs. 25.9%: bovine; 14.4% vs. 7.2%).

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, University of Maine, Hitchner Hall, 04469, Orono, ME, USA

    Rosalita M Smagula, Charles E Moody & Anne P Sherblom

  2. Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602, Athens, GA, USA

    Herman Van Halbeek

  3. Metabolism Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 6B09, 20892, Bethesda, MD, USA

    Jean M Decker & Andrew V Muchmore

Authors
  1. Rosalita M Smagula
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  2. Herman Van Halbeek
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  3. Jean M Decker
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  4. Andrew V Muchmore
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  5. Charles E Moody
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  6. Anne P Sherblom
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Smagula, R.M., Van Halbeek, H., Decker, J.M. et al. Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine uromodulin (Tamm-Horsfall glycoprotein). Glycoconjugate J 7, 609–624 (1990). https://doi.org/10.1007/BF01189081

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  • DOI: https://doi.org/10.1007/BF01189081

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