Summary
In the adult human mammary gland, most of the luminal epithelial cells express keratin 19 (K19+). However, in some small ducts and terminal ductal lobular units where branching would be expected to occur during pregnancy, the pattern of expression of this keratin is heterogeneous. While the keratin 19 negative cells (K19−) appear to have a high proliferative potentialin vitro andin vivo, they have a lower secretory activity than the K19+ cells as monitored by expression of secretory component in the resting breast or casein in the pregnant gland.
That the K19− cells form a separate proliferative compartment in the luminal cell lineage is suggested by the fact that they are absent in the prepubertal breast and only appear at puberty associated with branching ducts, and newly formed lobules. Our observations are consistent with the hypothesis that the K19− luminal cell is less differentiated than and may be precursor to the K19+ luminal cell, which represents the fully differentiated phenotype able to produce milk in response to a hormonal stimulus.
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Bartek, J., Viklicky, V., Franek, F., Angelisova, P., Draber, P., Jarosikova, T., Nemec, M. &Verlova, H. (1982) Monoclonal antibodies against transferrin. Precipitating mixtures and lack of interspecies crossreactivity.Immunol. Lett. 4, 231–5.
Bartek, J., Durban, E. M., Hallowes, R. C. &Taylor-Papadimitriou, J. (1985a) A subclass of liminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins.J. Cell. Sci. 75, 17–33.
Bartek, J., Taylor-Papadimitriou, J., Miller, N. &Millis, R. (1985b) Patterns of expression of keratin 19 as detected with monoclonal antibodies in human tissues and tumours.Int. J. Cancer 36, 299–306.
Bartek, J., Bartkova, J., Schneider, J., Taylor-Papadimitriou, J., Kovarik, J. &Rejthar, A. (1986a) Expression of monoclonal antibody-defined epitopes of keratin 19 in human tumours and cultured cells.Eur J. Cancer Clin. Oncol.,22, 1441–52.
Bartek, J., Bartkova, J., Taylor-Papadimitriou, J., Rejthar, A., Kovarik, J., Lukas, Z. &Vojtesek, B. (1986b) Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies.Histochem. J.,18, 565–75.
Bartek, J., Kovarik, J., Burchell, J., Taylor-Papadimitrious, J., Bartkova, J., Vojtesek, B., Rejthar, A., Schneider, J., Petrek, M., Staskova, Z., &Millis, R. (1987) Monoclonal antibodies to breast epithelial antigens in the study of differentiation and malignancy. InMolecular Biology and Differentiation of Cancer Cells vol. 2 (edited byLapis, K. &Eckhardt, S.) pp. 123–30. Budapest: Karger, Basel/Akademiai Kiado.
Bartek, J., Tlaskalova-Hogenova, H., Staskova, Z., Simeckova, J., Vojtesek, B., Rejthar, A., Kovarik, J. &Bartkova, J. (1989) Secretory component in differentiating normal epithelium, benign lesions and malignancy in the human breast as monitored by monoclonal antibodies.Histochem. 91, 235–44.
Burchell, J., Durbin, H. &Taylor-Papadimitriou, J. (1983) Complexity of expression of antigenic determinants recognised by monoclonal antibodies HMFG-1 and HMFG-2, in normal and malignant human mammary epithelial cells.J. Immunol. 131, 508–13.
Dubois, J.-D., O'Hare, M. J., Monaghan, P., Bartek, J., Norris, R. &Gusterson, B. A. (1987) Human breast epithelial xenografts: an immunocytochemical and ultrastructural study of differentiation and lactogenic response.Differentiation,35, 72–82.
Dulbecco, R., Allen, W. R., Bologna, M. &Bowman, M. (1986) Marker evolution during the development of the rat mammary gland: stem cells identified by markers and the role of myoepithelial cells.Cancer Res. 46, 2449–56.
Gendler, S., Taylor-Papadimitriou, J., Duhig, T., Rothbard, J. &Burchell, J. (1988) A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats.J. Biol. Chem. 263, 12820–3.
Gugliotta, P., Sapino, A., Macri, L., Skalli, O., Gabbiani, G. &Bussolati, G. (1988) Specific demonstration of myoepithelial cells by anti-alpha smooth muscle actin antibody.J. Histochem. Cytochem. 36, 659–63.
Gusterson, B. A., Sloane, J. P., Middwood, C., Gazet, J. C., Trott, P., Taylor-Papadimitriou, J. &Bartek, J. (1987) Ductal adenoma of the breast-a lesion exhibiting a myoepithelial/epithelial phenotype.Histopathol. 11, 103–10.
Hammond, S. L., Ham, R. C. &Stampfer, M. R. (1984) Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract.Proc. natl. Acad. Sci. USA 81, 5435–9.
Karsten, U., Papsdorf, G., Roloff, G., Stolley, P., Abel, H., Walther, I., &Weiss, H. (1985) Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.Eur. J. Cancer Clin. Oncol. 21, 733–40.
Lane, E. B. (1982) Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization.J. Cell Biol. 92, 665–73.
Lauerova, L., Kovarik, J., Bartek, J., Rejthar, A. &Vojtesek, B. (1988) Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule.Hybridoma,7, 495–504.
MacMahon, B., Cole, P., Lin, T. M., Lowe, C. R., Mirra, A. P., Ravnihar, B., Salber, E. J., Valaoras, V. G. &Yuasa, S. (1970) Age at first birth and breast cancer risk.Bull. Wld. Hlth. Org. 43, 209–21.
McGregor, D. H., Land, C. E., Choi, K., Tokuoka, S., Liu, P. I., Wakabayashi, T. &Beebe, G. W. (1977) Breast cancer incidence among atomic bomb survivors, Hiroshima and Nagasaki, 1950–69.J. Natl. Cancer Inst. 59, 799–811.
Nagle, R. B., Boecker, W., Davis, J. R., Heid, H. W., Kaufmann, M., Lucas, D. O. &Jarasch, E.-D. (1986) Characterisation of breast carcinomas by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells.J. Histochem. Cytochem. 34, 869–81.
Russo, J. &Russo, I. H. (1987) Development of the human mammary gland. InThe Mammary Gland (edited byNeville, M. C. &Daniel, C. W.), pp. 67–93. Plenum Publishing Corporation.
Shimizu, M. &Yamauchi, K. (1982) Isolation and characterization of mucin-like glycoproteins in human mild fat globule membranes.J. Biochem. 91, 515–19.
Skalli, D., Ropraz, P., Trzeciak, A., Benzonana, G., Gillesen, D. &Gabbiani, G. (1986) A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation.J. Cell Biol. 103, 2787–96.
Smith, C. A., Monaghan, P. &Neville, A. M. (1984) Basal clear cells of the normal human breast.Virchows Arch. (Pathol. Anat.) 402, 319–29.
Stampfer, M., Hallowes, R. C. &Hackett, A. J. (1980) Growth of normal human mammary cells in culture.In vitro 16, 415–25.
Taylor-Papadimitriou, J. &Fentiman, I. S. (1981) Culture of cells from human breast: problems and potentials. InCommentaries on Research in Breast Disease (edited byBulbrook, R. D. &Taylor, D. J.) Vol. 2, pp. 87–107, New York: A. R. Liss.
Taylor-Papadimitriou, J. & Lane, E. B. (1987) Keratin expression in the mammary gland. InThe Mammary Gland (edited byNeville, M. C. & Daniel, C. W.), pp. 181–215, Plenum Publishing Corporation.
Taylor-Papadimitriou, J., Lane, E. B. &Chang, S. E. (1983) Cell lineages and interactions in neoplastic expression in the human breast. InUnderstanding Breast Cancer: Clinical and Laboratory Concepts (edited byRich, M. A., Hager, J. C. &Furmanski, P.), pp. 215–46, New York: Marcel Dekker, Inc.
Taylor-Papadimitriou, J., Stampfer, M., Bartek, J., Lewis, A., Lane, E. B. &Leigh, I. M. (1989) Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation toin vivo phenotypes and influence of medium.J. Cell Sci. 94, 403–13.
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Bartek, J., Bartkova, J. & Taylor-Papadimitriou, J. Keratin 19 expression in the adult and developing human mammary gland. Histochem J 22, 537–544 (1990). https://doi.org/10.1007/BF01005976
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DOI: https://doi.org/10.1007/BF01005976