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Effect of food restriction on serotonin metabolism in rat brain

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Abstract

The brain concentration of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) increased in rats maintained on restricted volume of low-protein or normal-protein diet, whereas these two agents decreased in rats fed low-protein diet ad libitum. In these two food-restricted groups brain 5-HT and 5-HIAA concentrations were not correlated with brain tryptophan hydroxylase activity, but the concentrations correlated closely with cerebral tryptophan concentrations. The cerebral tryptophan concentration in the two food-restricted groups was not consistent with the total or free tryptophan concentration in plasma. In these restricted rats cerebral tryptophan concentration was elevated, and, unlike the plasma tryptophan, it showed no diurnal variation. These results suggested that tryptophan uptake into the brain from plasma was enhanced by limiting food volume intake. Tryptophan uptake was increased by glucagon injection without changing the plasma tryptophan level, but injection of hydrocortisone or insulin had little or no effect on tryptophan concentration in either the plasma or brain.d-Glucose injection elevated plasma tryptophan concentration but decreased brain tryptophan concentration.

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References

  1. Dickerson, J. W. T., andPao, S. K. 1975. The effect of a low protein diet and exogenous insulin on brain tryptophan and its metabolism in the weanling rat.J. Neurochem. 25:559–564.

    PubMed  Google Scholar 

  2. Ahmud, G., andRahman, M. A. 1975. Effects of undernutrition and protein malnutrition on brain chemistry of rats.J. Nutr. 105:1090–1103.

    PubMed  Google Scholar 

  3. Ramanamurthy, P. S. V. 1977. Maternal and early postnatal malnutrition and transmitter amines in rat brain.J. Neurochem. 28:253–254.

    PubMed  Google Scholar 

  4. Tsukada, Y., Kohsaka, S., andNagai, K. 1979. Effect of mild protein restriction during pre and postnatal development of the rats on discriminative learning ability.In Brozek, J. (ed.),Behavioral Effects of Energy and Protein Deficits, NIH Publication, in press.

  5. Fischer, C. A., Kariya, T., andAprison, M. H. 1970. A comparison of the distribution of 5-hydroxyindole acetic acid and 5-hydroxytryptamine in four specific brain areas of the rat and pigeon.Comp. Gen. Pharmacol. 1:61–68.

    PubMed  Google Scholar 

  6. Denckla, W. D., andDewey, H. K. 1967. The determination of tryptophan in plasma, liver, and urine.J. Lab. Clin. Med. 69:160–169.

    PubMed  Google Scholar 

  7. Madras, B. K., Cohen, E. L., Messing, R., Munro, H. N., andWurtman, R. J. 1974. Relevance of free tryptophan in serum to tissue tryptophan concentration.Metabolism 23:1107–1116.

    PubMed  Google Scholar 

  8. Gal, E. M., andPatterson, K. 1973. Rapid nonisotopic assay of tryptophan 5-hydroxylase activity in tissues.Anal. Biochem. 52:625–629.

    PubMed  Google Scholar 

  9. Hultman, E. 1959. Rapid specific method for determination of aldosaccharides in body fluids.Nature 183:108–109.

    PubMed  Google Scholar 

  10. Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265–275.

    PubMed  Google Scholar 

  11. Jequier, E., Lovenberg, W., andSjoerdsma, A. 1967. Tryptophan hydroxylase inhibition; the mechanism by whichp-chlorophenylalanine depletes rat brain serotonin.Mol. Pharmacol. 3:274–278.

    PubMed  Google Scholar 

  12. Moir, A. T. B., andEccleston, D. 1968. The effects of precursor loading in the central metabolism of 5-hydroxyindoles.J. Neurochem. 15:1093–1108.

    PubMed  Google Scholar 

  13. Eccleston, D., Ashcroff, G. W., andCrawford, T. B. B. 1965. 5-Hydroxyindole metabolism in rat brain; a study of intermediate metabolism using the technique of tryptophan loading II.J. Neurochem. 12:493–503.

    PubMed  Google Scholar 

  14. Kaufman, S. 1974. Properties of pterin-dependent aromatic amino acid hydroxylases. Pages 85–108,in Wolstenholine, G. E. W., andFitzsimons, D. W. (eds.)Aromatic Amino Acid in the Brain, Elsevier, North-Holland, Amsterdam.

    Google Scholar 

  15. Fernstrom, J. D., andWurtman, R. J. 1971. Brain serotonin content; increase following ingestion of carbohydrate diet.Science 174:1023–1025.

    PubMed  Google Scholar 

  16. Fernstrom, J. D., andWurtman, R. J. 1971. Brain serotonin content; physiological dependence on plasma tryptophan levels.Science 173:149–152.

    PubMed  Google Scholar 

  17. Mcmenamy, R. H., Lund, C. C., andOncley, J. L. 1957. Unbound amino acid concentrations in human blood plasma.J. Clin. Invest. 36:1672–1679.

    PubMed  Google Scholar 

  18. Mcmenamy, R. H., andOncley, J. L. 1958. Specific binding ofl-tryptophan to serum albumin.J. Biol. Chem. 233:1436–1447.

    PubMed  Google Scholar 

  19. Knott, P. J., andCurzon, G. 1972. Free tryptophan in plasma and brain tryptophan metabolism.Nature 239:452–453.

    PubMed  Google Scholar 

  20. Curzon, G., Kantamaneni, B. D., Winah, J., Rojas-Bueno, A., Murray-Lyon, I. M., andWilliams, R. 1973. Plasma and brain tryptophan changes in experimental acute hepatic failure,J. Neurochem. 21:137–145.

    PubMed  Google Scholar 

  21. Perez-Cruet, J., Tagliamonte, A., Tagliamonte, P., andGessa, G. L. 1972. Changes in brain serotonin metabolism associated with fasting and satiation in rats.Life Sci. 11:31–39.

    Google Scholar 

  22. Curzon, G., Joseph, M. H., andKnott, P. J. 1972. Effects of immobilization and food deprivation on rat brain tryptophan metabolism.J. Neurochem. 19:1967–1974.

    PubMed  Google Scholar 

  23. Mans, A. M., Saunders, S. J., Kirsch, D. E., andBiebuyek, J. F. 1979. Correlation of plasma and brain amino acid and putative neurotransmitter alterations during acute hepatic coma in the rat.J. Neurochem. 32:285–292.

    PubMed  Google Scholar 

  24. Fernstrom, J. D., Larin, F., Schonfeld, G., andWurtman, R. J. 1971. Insulin-induced changes in plasma tryptophan in rats and man.Fed. Proc. 30:250.

    Google Scholar 

  25. Fernstrom, J. D., andWurtman, R. J. 1972. Elevation of plasma tryptophan by insulin in rat.Metabolism 21:337–342.

    PubMed  Google Scholar 

  26. Fernstrom, J. D., Hirsch, M. J., Madras, B. K., andSudarsky, L. 1975. Effects of skim milk, whole milk and light cream on serum tryptophan binding and brain tryptophan concentrations in rats.J. Nutr. 105:1359–1362.

    PubMed  Google Scholar 

  27. Fernando, J. C. R., Knott, P. J., andCurzon, G. 1976. The relevance of both free tryptophan and insulin to rat brain tryptophan concentration.J. Neurochem. 27:343–345.

    PubMed  Google Scholar 

  28. Montis, M. G. D., Olianas, M. C., Haber, B., andTagliamonte, A. 1978. Increase in large amino acid transport into brain by insulin.J. Neurochem. 30:121–124.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, 160, Shinjuku-ku, Tokyo, Japan

    S. Kohsaka, K. Takamatsu & Y. Tsukada

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  1. S. Kohsaka
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  2. K. Takamatsu
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  3. Y. Tsukada
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Kohsaka, S., Takamatsu, K. & Tsukada, Y. Effect of food restriction on serotonin metabolism in rat brain. Neurochem Res 5, 69–79 (1980). https://doi.org/10.1007/BF00964461

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