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Severe pulmonary pneumocystosis in simian acquired immunodeficiency syndrome induced by simian immunodeficiency virus: its characterization by the polymerase-chain-reaction method and failure of experimental transmission to immunodeficient animals

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Abstract

Pneumocystis carinii (Pc) infection was observed in three of five rhesus monkeys infected with simian immunodeficiency virus (SIVmac251). They showed severe symptoms similar to those associated with human acquired immunodeficiency syndrome (AIDS). Histopathology revealed severe pulmonary pneumocystosis in one of three Pc-positive monkeys, and anti-Pc antibodies were detected in sera from two of the three monkeys. Localization of Pc organisms in various organs of the monkeys was examined by the polymerase-chain-reaction (PCR) method, and Pc-specific bands of DNA amplification were detected in the liver, kidney, spleen, adrenal gland, testis, brain, and other organs examined, but no Pc organism was found in these organs by histopathologic examination. These results suggest that the activation of a latent infection of Pc occurs in SIV-infected rhesus monkeys as well as in human AIDS. Experimental transmission of Pc derived from a simian was attempted in severe combined immunodeficiency (SCID) mice and athymic nude (rnu/rnu, F344) rats. These animals were inoculated intranasally with 104 Pc cysts, but neither histopathologic changes nor Pc organisms were detected in SCID mice at 4 months after inoculation or in nude rats at 2 months postinoculation, suggesting that simian Pc is species-specific.

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Authors and Affiliations

  1. Department of Parasitology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108, Tokyo, Japan

    T. Furuta & S. Kojima

  2. Second Department of Veterinary Public Health, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, 229, Sagamihara, Kanagawa, Japan

    M. Fujita

  3. Tsukuba Primate Center for Medical Science, National Institute of Health of Japan, 1 Hachimandai, 305, Tsukuba, Japan

    R. Mukai, I. Sakakibara & Y. Yoshikawa

  4. Department of Pathology, National Institute of Health of Japan, 1-23-1, 162, Toyama, Shinjuku-ku, Japan

    T. Sata

  5. Tonen Corporate Research & Development Laboratory, 1-3-1 Nishi-Tsurugaoka Ohi-Machi, Iruma-Gun, 354, Saitama, Japan

    K. Miki

  6. Virus Institute, Kyoto University, 53 Kawara-machi, Seigoin, Sakyo-ku, 606, Kyoto, Japan

    M. Hayami

Authors
  1. T. Furuta
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  2. M. Fujita
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  3. R. Mukai
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  4. I. Sakakibara
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  5. T. Sata
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  6. K. Miki
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  7. M. Hayami
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  8. S. Kojima
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  9. Y. Yoshikawa
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Furuta, T., Fujita, M., Mukai, R. et al. Severe pulmonary pneumocystosis in simian acquired immunodeficiency syndrome induced by simian immunodeficiency virus: its characterization by the polymerase-chain-reaction method and failure of experimental transmission to immunodeficient animals. Parasitol Res 79, 624–628 (1993). https://doi.org/10.1007/BF00932502

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