Abstract
Sites hypersensitive to digestion by DNase I have been identified within the 5′-flanking and 3′-coding sequences of genes encoding the defense enzyme chalcone synthase in bean (Phaseolus vulgaris L.). Two of the 5′-flanking hypersensitive sites are markedly induced upon elicitation of cells with glutathione and delineate sequence elements that are also present in the promoters of coordinately regulated genes. In contrast, other hypersensitive sites within the 5′-flanking sequences are expressed constitutively and one maps within an element that is also present in the promoters of coordinately regulated genes. These results suggest that the transcriptional activation of chalcone synthase genes is accompanied by structural changes in the chromatin associated with the proximal region of the promoter and that these probably reflect the binding of transcription factors tocis-regulatory elements.
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Ashraf, M., Vasil, V., Vasil, I., and Ferl, R.J. (1987)Mol. Gen. Genet. 208, 185–190.
Cramer C.L., Edwards, K., Dron, M., Liang, X., Dildine, S.L., Bolwell, G.P., Dixon, R.A., Lamb, C.J., and Schuch, W. (1989)Plant Mol. Biol. 12, 367–383.
Dron, M., Clouse, S.D., Dixon, R.A., Lawton, M.A., and Lamb, C.J. (1988)Proc. Natl. Acad. Sci. USA 85, 6738–6742.
Elgin, S.C.R. (1989)J. Biol. Chem. 263, 19259–19262.
Emerson, B.M., and Felsenfeld, G. (1984)Proc. Natl. Acad. Sci. USA 81, 95–99.
Hedrick, S.A., Bell, J.N., Boller, T., and Lamb, C.J. (1988)Plant Physiol. 86, 182–186.
Kaye, J.S., Bellard, M., Dretzen, G., Bellard, F., and Chambon, P. (1984)EMBO J. 3, 1137–1144.
Kuhlemeier, C., Cuozzo, M., Green, P.J., Goyvaerts, E., Ward, K., and Chua, N-H. (1988)Proc. Natl. Acad. Sci. USA 85, 4662–4666.
Lamb, C.J., Lawton, M.A., Dron, M., and Dixon R.A. (1989)Cell 56, 215–224.
Lawton, M.A., and Lamb, C.J. (1987)Mol. Cell. Biol. 7, 335–341.
Lawton, M.A., Dixon, R.A., Hahlbrock, K., and Lamb, C.J. (1983)Eur. J. Biochem. 130, 131–139.
Lois, R., Dietrich, A., Hahlbrock, K. and Schulz, W. (1989)EMBO J. 8, 1641–1648.
Luthe, D.S., and Quatrano, R.S. (1980)Plant Physiol. 65, 305–308.
Meinkoth, J. and Wahl, G. (1984)Anal. Biochem. 138, 267–284.
Paul, A-L., Vasil, V., Vasil, I., and Ferl, R. (1987)Proc. Natl. Acad. Sci. USA 84, 799–803.
Riven, C.J., Zimmer, E.A., and Walbot, V. (1982) InMaize for Biological Research, W.F. Sheridan, ed. (Charlottesville, VA: Plant Molecular Biology Association) pp 161–164.
Ryder, T.B., Hedrick, S.A., Bell, J.N., Liang, X., Clouse, S.D., and Lamb, C.J. (1987)Mol. Gen. Genet. 210, 219–233.
Thomas, G.H., Seigfreid, E., and Elgin, S.C.R. (1986) InChromosomal Proteins and Gene Expression, G. Reeck, G. Goodwin and P. Pvigdomenech, eds (New York, N.Y: Plenum Press) pp 77–101.
Weiher, H., Konig, M., and Gruss, P. (1983)Science 219, 626–631.
Weisbrod, S. (1982)Nature 297, 289–295.
Wingate, V.P.M., Lawton, M.A., and Lamb, C.J. (1988)Plant Physiol. 87, 206–210.
Zaret, K.S., and Yamamoto, K.R. (1984)Cell 38, 29–38.
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Communicated by J. M. Widholm
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Lawton, M.A., Clouse, S.D. & Lamb, C.J. Glutathione-elicited changes in chromatin structure within the promoter of the defense gene chalcone synthase. Plant Cell Reports 8, 561–564 (1990). https://doi.org/10.1007/BF00820210
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DOI: https://doi.org/10.1007/BF00820210