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Dynamic changes in flow rate and composition of urine during the post-bloodmeal diuresis inAedes aegypti (L.)

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Summary

  1. 1.

    The urine of blood-fed mosquitos was collected and analyzed for elemental composition and osmolality.

  2. 2.

    Peak rates of urine flow averaged 4.9 droplets/min at 6 min following the beginning of the bloodmeal; peak flow urine contained, in mM, Na 178, K 4, and Cl 132, and the urine was approximately isosmotic to hemolymph.

  3. 3.

    As urine flow rates fell, the [Na] of the urine decreased and the [K] increased. Urine osmolality declined, measuring less than 100 mOsm/kg in some samples, as compared to 354 mOsm/kg measured in pre-bloodmeal hemolymph.

  4. 4.

    When urine flow rates had fallen to less than 10% of peak flow rates, urine again became approximately isosmotic to hemolymph, still containing Na and K as the principal cations.

  5. 5.

    Approximately 40% each of the water, Na and Cl contained in the plasma fraction of the bloodmeal was excreted during the 1–2h period following the bloodmeal. This excretion represents only 20–30% of the total loads ingested in the bloodmeal.

  6. 6.

    The data are consistent with dynamic changes in the functioning of both the Malpighian tubules and hindgut during the post-bloodmeal diuresis.

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References

  • Aston RJ (1975) The role of adenosine 3′:5′-cyclic monophosphate in relation to the diuretic hormone ofRhodnius prolixus. J Insect Physiol 21:1873–1877

    Google Scholar 

  • Bonventre JV, Blouch K, Lechene C (1981) Liquid droplets and isolated cells. In: Mazat MA (ed) X-ray microscope in biology, University Park Press, Baltimore, pp 307–366

    Google Scholar 

  • Boorman JPT (1960) Observations on the feeding habits of the mosquitoAedes (Stegomyia) aegypti (Linnaeus): the loss of fluid after a blood-meal and the amount of blood taken during feeding. Ann Trop Med Parasitol 54:8–14

    Google Scholar 

  • Christophers SR (1960)Aedes aegypti (L.) the yellow fever mosquito. Cambridge University Press, London, pp 468, 489

    Google Scholar 

  • Clements AN (1963) The physiology of mosquitoes. Pergamon Press, New York, pp 159–161

    Google Scholar 

  • Florkin M, Jeuniaux C (1974) Hemolymph: composition. In: Rockstein M (ed) The physiology of insecta, 2nd edn, vol 5. Academic Press, New York London, pp 255–307

    Google Scholar 

  • Gee JD (1975) Diuresis in the tsetse flyGlossina austeni. J Exp Biol 63:381–390

    Google Scholar 

  • Gee JD (1977) The hormonal control of excretion. In: Gupta BL, Moreton RB, Oschman JL, Wall BJ (eds) Transport of ions and water in animals. Academic Press, London New York San Francisco, pp 265–281

    Google Scholar 

  • Hanaoka K, Hagedorn HH (1980) Brain hormone control of ecdysone secretion by the ovary in a mosquito. In: Hoffman JA (ed) Progress in ecdysone research. Elsevier, Amsterdam, pp 467–480

    Google Scholar 

  • Howard LM (1962) Studies on mechanism of infection of the mosquito midgut byPlasmodium gallinaceum. Am J Hyg 75:287–300

    Google Scholar 

  • Mack SR, Vanderberg JP (1978) Hemolymph ofAnopheles stephensi from noninfected andPlasmodium berghei-infected mosquitoes. I. Collection procedure and physical characteristics. J Parasitol 64:918–923

    Google Scholar 

  • Maddrell SHP, Phillips JE (1978) Induction of sulfate transport and hormonal control of fluid secretion by Malpighian tubules of larvae of the mosquitoAedes taeniorhynchus. J Exp Biol 72:181–202

    Google Scholar 

  • Nijhout HF, Carrow GM (1978) Diuresis after a bloodmeal in femaleAnopheles freeborni. J Insect Physiol 24:293–298

    Google Scholar 

  • Phillips JE (1981) Comparative physiology of insect renal function. Am J Physiol 241:R241-R257

    Google Scholar 

  • Pilitt DR, Jones JC (1972) A qualitative method for estimating the degree of engorgement ofAedes aegypti adults. J Med Entomol 9:334–337

    Google Scholar 

  • Roinel N (1975) Electron microprobe quantitative analysis of lyophilized 10−10 l volume samples. J Microsc (Paris) 22:261–268

    Google Scholar 

  • Shapiro JP, Hagedorn HH (1982) Juvenile hormone and the development of ovarian responsiveness in the mosquito,Aedes aegypti. Gen Comp Endocrinol 46:176–183

    Google Scholar 

  • Stobbart RH (1977) The control of diuresis following a bloodmeal in females of the yellow fever mosquitoAedes aegypti (L.). J Exp Biol 69:53–85

    Google Scholar 

  • Wigglesworth VB (1931) The physiology of excretion in a bloodsucking insect,Rhodnius prolixus (Hemiptera, Reduviidae) I. Composition of the urine. J Exp Biol 8:411–427

    Google Scholar 

  • Williams JC, Beyenbach KW (1983) Differential effects of secretagogues on Na and K secretion in the Malpighian tubules ofAedes aegypti (L.). J Comp Physiol 149:511–517

    Google Scholar 

  • Winogradskaja ON (1936) Osmotischer Druck der Hämolymphe beiAnopheles maculipennis messeae Fall. Z Parasitenkd 8:697–713

    Google Scholar 

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Author notes

  1. James C. Williams Jr.

    Present address: Nephrology Research and Training Center, University of Alabama in Birmingham, 35294, Birmingham, Alabama, USA

Authors and Affiliations

  1. Section of Physiology, Division of Biological Sciences and Department of Entomology, Cornell University, 14853, Ithaca, New York, USA

    James C. Williams Jr., Henry H. Hagedorn & Klaus W. Beyenbach

Authors
  1. James C. Williams Jr.
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  2. Henry H. Hagedorn
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  3. Klaus W. Beyenbach
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Williams, J.C., Hagedorn, H.H. & Beyenbach, K.W. Dynamic changes in flow rate and composition of urine during the post-bloodmeal diuresis inAedes aegypti (L.). J Comp Physiol B 153, 257–265 (1983). https://doi.org/10.1007/BF00689629

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  • DOI: https://doi.org/10.1007/BF00689629

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