Summary
Beta-glucuronidase activity has been studied in 46 cerebral tumors by Naphthol AS-BI beta-D-glucuronide and diazotized pararosanilin. The distribution pattern of the enzyme activity is similar to that already observed for other lysosomal enzymes, with some minor differences. The histochemical staining increases in regressive or catabolic areas so that its relationship with malignancy appears to be indirect. Special regard is devoided to some cytotypes, such as giant cells of glioblastoma and gemistocytic astrocytes whose high enzyme activity is considered as a sign of cellular regression.
Zusammenfassung
Die Aktivität der Beta-Glucuronidase wurde in 46 Hirngeschwülsten mittels Naphthol-AS-BI-beta-D-Glucuronid und diazotiertem Pararosanilin untersucht. Das Verteilungsmuster der Enzymaktivität ist ähnlich dem bereits für andere lysosomale Enzyme beobachteten, zeigt aber leichte Abweichungen. Die histochemische Anfärbung nimmt in regressiven und katabolen Gebieten zu, so daß eine indirekte Beziehung zur Malignität zu bestehen scheint. Besonderes Augenmerk wird einigen Zelltypen zugewandt, wie den Riesenzellen im Glioblastom sowie gemästeten Astrocyten, deren hohe Enzymaktivität als Zeichen einer regressiven Zellreaktion gewertet wird.
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Schiffer, D., Fabiani, A., Cognazzo, A. et al. A histochemical study on the distribution and localization of Beta-glucuronidase activity in cerebral tumors. Acta Neuropathol 13, 91–96 (1969). https://doi.org/10.1007/BF00686143
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DOI: https://doi.org/10.1007/BF00686143