Summary
A human oligodendroglial cell line has been established from a mixed glioma. Over a period of 22 months, the cell consisted of cells with the morphologic features of such a strong perinuclear halo, dichotomous branching, and a tendency to aggregate and float in the medium. In small aggregates, most cells are bipolar. The cell line also continues to synthetize the S-100 protein specific to neural tissue. The response of this cell line to dibutyryl-cyclic AMP is striking, resulting in a remarkable narrowing and elongation of the cell processes.
This cell line could be used for studies on glial differentiation and neuron-glia interaction.
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This work was supported by a Grant-in Aid for Scientific Research from the Japan Ministry of Education, Science, and Culture
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Miyake, E. Establishment of a human oligodendroglial cell line. Acta Neuropathol 46, 51–55 (1979). https://doi.org/10.1007/BF00684804
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DOI: https://doi.org/10.1007/BF00684804