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Coordinate change in phenotype in a mouse cell line selected forCD8 expression

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Abstract

A CD4+, CD8+ derivative of the CD4+, CD8− cell line SAKRTLS 12.1 was isolated by fluorescence activated cell sorting for CD8+ cells. This derivative showed a co-ordinate change in a number of independent characters: The parental cell line was CD4+, CD8−, CD3+, CD5hi, HSA+, DEXR, CD44hi, while the derivative was CD4+, CD8+, CD3−, CD510, HSA+, DEXS, CD4410. The derivative expressed the Thy-1.1, Ly-2.1, and Ly-3.1 surface antigens, consistent with origin from the SAKRTLS 12.1 parental cell line, and showed a drug resistance profile identical to that of the parent. It was not possible to isolate revertants with a phenotype identical to that of the parental cell line. Activation of the structural gene coding for CD8 α chain was correlated with demethylation at several sites. We interpret these results to mean that this CD8+ derivative of SAKRTLS 12.1 arose as a result of an alteration of a gene that coordinately regulates multiple genes whose expression changes during thymocyte differentiation. Gene methylation may contribute, directly or indirectly, to some or all of the changes in gene expression observed.

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  1. Cancer Biology Laboratory, The Salk Institute, Box 85800, 92186-5800, San Diego, CA, USA

    Robert Hyman & Valerie Stallings

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  1. Robert Hyman
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Hyman, R., Stallings, V. Coordinate change in phenotype in a mouse cell line selected forCD8 expression. Immunogenetics 36, 149–156 (1992). https://doi.org/10.1007/BF00661091

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