Abstract
The Ca2+ and Sr2+ activation of tension in functionally skinned chicken fibers of normal and dystrophic skeletal and normal cardiac muscle were studied. The muscles studied can be separated into two groups based upon their Ca2+ and Sr2+ sensitivities: those which are significantly more sensitive to Ca2+ than to Sr2+, pectoralis and posterior latissimus dorsi (PLD), and those which show no Ca2+/Sr2+ sensitivity difference, cardiac and anterior latissimus dorsi (ALD). This suggests that there is more than one type of Ca2+ site involved in Ca2+ control of muscle contraction in different muscle types and suggests that ALD and cardiac muscle may be controlled by a different type of binding site than PLD and pectoralis muscle. Dystrophic ALD and PLD muscles showed little change in their Ca2+ and Sr2+ sensitivities from those of normal muscles in contrast to the pectoralis which showed a decrease in both Ca2+ and Sr2+ sensitivity (approaching that of PLD) with the onset of dystrophy. Similarly, upon SDS polyacrylamide gel electrophoresis, dystrophic ALD and PLD muscles showed no difference in contractile proteins from those of normal muscles, in contrast to pectoralis muscle where the appearance of a 36,000 dalton protein band correlated with the onset of dystrophy and the changes in the Ca2+/Sr2+ activation properties of this muscle. The contractile protein band pattern of normal and dystrophic PLD and dystrophic pectoralis muscle were similar including the presence of the 36,000 dalton protein.
Similar content being viewed by others
References
Amphlett G. W., Perry, S. V., Syska, H., Brown, M. D., Vrbova, G.: Cross innervation and the regulatory protein system of rabbit soleus muscle. Nature257, 602–604 (1975)
Bárány, M., Gaetjens, E., Bárány, K.: Myosin in hereditary muscular dystrophy of chickens. Ann. N. Y. Acad. Sci.138, 360–366 (1966)
Berson, G.: The “γ component” of skeletal troponin: Evidence for its identity with muscle creatine kinase. J. Biol. Chem.251 (22), 7001–7003 (1976)
Best, P. M., Donaldson, S. K. B., Kerrick, W. G. L.: Tension in mechanically disrupted mammalian cardiac cells: Effects of magnesium adenosine triphosphate. J. Physiol. (Lond.)265, 1–17 (1977)
Dayton, W. R., Reville, W. J., Goll, D. E., Stromer, M. H.: A Ca2+-activated protease possibly involved in myofibrillar protein turnover. Partial characterization of the purified enzyme. Biochemistry15, 2159–2167 (1976)
Donaldson, S. K. B., Kerrick, W. G. L.: Characterization of the effects of Mg2+ on Ca2+- and Sr2+-activated tension generation of skinned skeletal muscle fibers. J. Gen. Physiol.66, 427–444 (1975)
Donaldson, S. K. B., Best, P. M., Kerrick, W. G. L.: Characterization of the effects of Mg2+ on Ca2+- and Sr2+-activated tension generation of skinned rat cardiac fibers. J. Gen. Physiol.71, 645–655 (1978)
Ebashi, S., Kodama, A., Ebashi, F.: Troponin. I. Preparation and physiological function. J. Biochem. (Tokyo)64, 465–477 (1968)
Ebashi, S., Endo, M., Ohtsuki, I.: Control of muscle contraction. Q. Rev. Biophys.2, 351–384 (1969)
Ebashi, S., Wakabashi, P., Ebashi, F.: Troponin and its components. J. Biochem. (Tokyo)69, 441–445 (1971)
Ebashi, S., Ohnishi, S., Abe, S., Maruyama, K.: Ca-dependent interaction of troponin components as the basis of the control mechanism by Ca ion. In: Calcium binding proteins. (W. Drabikowski, H. Strzelecka-Golaszewska, and E. Carafoli, eds.), pp. 179–196. Amsterdam-New York-Warsaw: Elsevier 1974
Greaser, M., Gergely, J.: Purification and properties of the components from troponin. J. Biol. Chem.248, 2125–2133 (1973)
Hellam, D. C., Podolsky, R. J.: Force measurements in skinned muscle fibers. J. Physiol. (Lond.)200, 807–819 (1969)
Ishiura, S., Murofushi, H., Suzuki, K., Imahori, K.: Studies of a calcium-activated neutral protease from chicken skeletal muscle. I. Purification and characterization. J. Biochem. (Tokyo)84, 225–230 (1978)
Kerrick, W. G. L., Best, P. M.: Calcium ion release in mechanically disrupted heart cells. Science183, 435–437 (1974)
Kerrick, W. G. L., Krasner, B.: Disruption of the sarcolemma of mammalian skeletal muscle fibers by homogenization. J. Appl. Physiol.39, 1052–1055 (1975)
Kerrick, W. G. L., Secrist, D., Coby, R., Lucas, S.: Development of difference between red and white muscles in sensitivity to Ca2+ in the rabbit from embryo to adult. Nature260, 440–441 (1976)
Kerrick, W. G. L., Hoar, P. E., Malencik, D. A., Pocinwong, S., Coby, R. L., Fischer, E. H.: Calcium ion activation: Characterization in skinned skeletal and cardiac muscle fibers. In: Proceedings third joint US-USSR symposium on myocardial metabolism, May 1977, pp. 195–209. National Institutes of Health 1978
Morey, K. S., Tarczy-Hornock, K., Brown, W. D.: Myosin from dystrophic and control chicken muscle. II. Molecular weight, electrophoretic properties, salt sensitivity, aggregation, and amino acid composition. Arch. Biochem. Biophys.124, 521–529 (1968)
Pelloni-Müller, G., Ermini, M., Jenny, E.: Changes in myosin light and heavy chain stoichiometry during development of rabbit fast, slow, and cardiac muscle. FEBS Lett.70, 113–117 (1976)
Porzio, M. A., Pearson, A. M.: Improved resolution of myofibrillar proteins with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Biochim. Biophys. Acta490, 27–34 (1977)
Roy, R. K., Potter, J. D., Sarkar, S.: Characterization of the Ca2+-regulatory complex of chick embryonic muscles: Polymorphism of tropomyosin in adult and embryonic fibers. Biochem. Biophys. Res. Commun.70, 28–36 (1976)
Su, J. Y., Kerrick, W. G. L.: Effects of halothane on caffeine-induced tension transients in the functionally skinned myocardial fibers. Pflügers Arch.380, 29–34 (1979)
Sugita, H., Toyokura, Y.: Alteration of troponin subunits in progressive muscular dystrophy (DMP). I. Pattern of troponin subunits in DMP. Proc. Jpn. Acad.52, 256–259 (1976)
Sugita, H., Toyokura, Y.: Alteration of troponin subunits in progressive muscular dystrophy (DMP). II. Mechanism of the alteration of troponin subunits in DMP. Proc. Jpn. Acad.52, 260–263 (1976)
VanEerd, J., Kawasaki, Y.: Effect of calcium (II) on the interaction between the subunits of troponin and tropomyosin. Biochemistry12, 4972–4980 (1973)
Weber, A., Murray, J. M.: Molecular control mechanisms in muscle contraction. Physiol. Rev.53, 612–673 (1973)
Weber, K., Osborn, M.: The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem.244, 4406–4412 (1969)
Weeds, A. G., Trentham, D. R., Kean, C. J., Buller, A. J.: Myosin from cross-reinnervated cat muscles. Nature247, 135–139 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kerrick, W.G.L., Hoar, P.E., Malencik, D.A. et al. Characterization of Ca2+- and Sr2+-activated tension in functionally skinned chicken fibers of normal and dystrophic skeletal and normal cardiac muscle. Pflugers Arch. 381, 53–62 (1979). https://doi.org/10.1007/BF00582332
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00582332