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The use of a carbodiimide-containing fixative for the immunohistochemical demonstration of coagulation factor VIII in rat vascular tissue

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Summary

Immunohistochemically, coagulation F-VIII-R:AG in vascular endothelial cells can be demonstrated with antihuman F-VIII-R:AG antisera after fixation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, using both peroxidase and fluorescence techniques. The generally used aldehyde containing fixatives, result in a loss of immunoreactivity. Carbodiimide is preferable as a fixative for the cellular localization of F-VIII-R:AG.

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Authors and Affiliations

  1. Gaubius Institute, Health Research Organization TNO, Herenstraat 5d, NL-2313 AD, Leiden, The Netherlands

    E. van Pelt-Verkuil & J. J. Emeis

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  1. E. van Pelt-Verkuil
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  2. J. J. Emeis
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van Pelt-Verkuil, E., Emeis, J.J. The use of a carbodiimide-containing fixative for the immunohistochemical demonstration of coagulation factor VIII in rat vascular tissue. Histochemistry 71, 187–194 (1981). https://doi.org/10.1007/BF00507823

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  • DOI: https://doi.org/10.1007/BF00507823

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