Abstract
Initial rate kinetics of methionine transport, time course of accumulation of methionine, and efflux of accumulated methionine were studied in three normal and four CF human diploid fibroblast strains. The range of apparent Km's was 12.7–32.1 μM for the CF strains and 18.3–39.2 μ M for the normal strains. The range of apparent Vmax's was 6.69–9.22 nmole mg−1 min−1 for the CF strains and 5.59–7.87 nmole mg−1 min−1 for the normal strains. The patterns of accumulation and efflux are quite similar in all the strains studied except for WI-38, which showed somewhat higher efflux and lower accumulation than the others. There was no significant difference in the kinetic parameters of methionine transport between CF and normal skin fibroblasts, and methionine transport will not serve as a marker for cystic fibrosis in cultured fibroblasts.
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This research was supported by a grant from the Cystic Fibrosis Foundation and a grant from the National Institutes of Health, Training Grant GMO1316 11 GNC.
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Sullivan, J.L., Kelly, J.C., Roess, W.B. et al. Membrane function in cystic fibrosis. II. Methionine transport in normal and cystic fibrosis fibroblasts. Biochem Genet 15, 1125–1132 (1977). https://doi.org/10.1007/BF00484502
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DOI: https://doi.org/10.1007/BF00484502