Abstract
Evidence is presented of the ability of H-2 class I antigens to function as teratocarcinoma transplantation (Gt) antigens. Coisogenic immunization against H-2 class I antigens expressed on transfected L cells is shown to induce resistance to embryonic carcinoma (EC) cell allografts. The Kb, Db, Dd, and, in appropriate recipients, Ld antigens can function as Gt antigens. The protocol presented may be useful for the molecular identification of other genes encoding histocompatibility antigens.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alonzo, A. D. R., Ferluga, J., Atfield, G., Santamaria, M., Fernandez, N., Garrido, A., Johnson, J., Schmidt, W., Mellor, A., Flavell, R. A., and Festenstein, H.: The expression of cloned class I H-2 b genes in L cells. Transplant. Proc. 15: 2111–2113, 1983
Artzt, K. and Jacob, F.: Absence of serologically detectable H-2 on primitive teratocarcinoma cells in culture. Transplantation 17: 632–634, 1974
Avner, P. R., Dove, W. F., Dubois, P., Gaillard, J. A., Guénet, J.-L., Jacob, F., Jakob, H., and Shedlovsky, A.: The genetics of teratocarcinoma transplantation: Tumor formation in allogeneic hosts by the embryonal carcinoma cell lines F9 and PCC3. Immunogenetics 7: 103–115, 1978
Croce, C. M., Linnenbach, A., Huebner, K., Parnes, J. R., Margulies, D. H., Apella, E., and Seidman, J. G.: Control of expression of histocompatibility antigens (H-2) and β 2 microglobulin in F9 teratocarcinoma stem cells. Proc. Natl. Acad. Sci. U.S.A. 78: 5754–5758, 1981
Goodenow, R. S., McMillan, M., Nicolson, M., Sher, B. T., Eakle, K., Davidson, N., and Hood, L.: Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer. Nature 300: 231–237, 1982
Goodenow, R. S., Stroynowski, I., McMillan, M., Nicolson, M., Eakle, K., Sher, B. T., Davidson, N., and Hood, L.: Expression of complete transplantation antigens by mammalian cells transformed with truncated class I genes. Nature 301: 388–394, 1983
Jakob, H., Boon, T., Gaillard, J., Nicolas, J. F., and Jacob, F.: Tératocarcinome de la souris: Isolement, culture et propriétés de cellules à potentialités multiples. Ann. Microbiol. 124 B: 269–282, 1974
Johnson, L. L. and Dove, W. F.: Male-specific transplantation antigen expression by XY teratocarcinomas PCC7 and 7′. Immunogenetics 19: 233–241, 1984
Johnson, L. L., Clipson, L. J., Dove, W. F., Feilbach, J., Maher, L. J., and Shedlovsky, A.: Teratocarcinoma transplantation antigens are encoded in the H-2 region. Immunogenetics 18: 137–145, 1983a
Johnson, L. L., Shedlovsky, A., Clipson, L. J., and Dove, W. F.: The genetics of allograft rejection of teratocarcinoma cell lines. In: Teratocarcinoma Stem Cells, Vol. 10, pp. 531–539, Cold Spring Harbor Conferences on Cell Proliferation, Cold Spring Harbor, New York 1983b
Klein, E. and Klein, G.: Specificity of homograft rejection in vivo, assessed by inoculation of artificially mixed compatible and incompatible tumor cells. Cell. Immunol. 5: 201–208, 1972
Kress, M., Glaros, D., Khoury, G., and Jay, G.: Alternative RNA splicing in expression of the H-2K gene. Nature 306: 602–604, 1983
Levine, A. and Teresky, A. K.: Teratocarcinoma transplantation rejection loci: Genetic localization of the Gt-1 locus on chromosome 17 and the expression of alternate alleles. Immunogenetics 13: 405–412, 1981
Maher III, L. J. and Dove, W. F.: Overt expression of H-2 serotypes on EC cells is not necessary for host rejection. Immunogenetics 19: 343–347, 1984
Mellor, A. L., Golden, L., Weiss, E., Bullman, H., Hurst, J., Simpson, E., James, R. F. L., Townsend, A. R. M., Taylor, P. M., Schmidt, W., Ferluga, J., Leben, L., Santamaria, M., Atfield, G., Festenstein, H., and Flavell, R. A.: Expression of murine H-2Kb histocompatibility antigen in cells transformed with cloned H-2 genes. Nature 298: 529–534, 1982
Morello, D., Gachelin, G., Dubois, Ph., Tanigaki, N., Pressman, D., and Jacob, F.: Absence of reaction of a xenogenic anti-H-2 serum with mouse embryonal carcinoma cells. Transplantation 26: 119–125, 1978
Morello, D., Daniel, F., Baldacci, P., Cayre, Y., Gachelin, G., and Kourilsky, P.: Absence of significant H-2 and β 2 microglobulin mRNA expression by mouse embryonal carcinoma cells. Nature 296: 260–262, 1982
Morris, R. E., Thomas, P. T., and Hong, R.: Cellular enzyme-linked immunospecific assay (CELISA): A new micromethod that detects antibodies to cell-surface antigens. Hum. Immunol. 5: 1–19, 1982
Murasko, D. M.: Apparent lack of H-2 restriction of allograft rejection. J. Immunol. 121: 958–961, 1978
Ostrand-Rosenberg, S., Cohn, A. L., and Sandoz, J. W.: Multiple splenic lymphoid cell subpopulations regulate H-2 antigen expression on teratocarcinoma cells in vivo. J. Immunol. 130: 2969–2973, 1983
Oudshoorn-Snoek, M., Démant, P., Mellor, A. L., and Flavell, R. A.: Antigenic characterization of products of cloned H-2 b genes. Transplant. Proc. 15: 2027–2032, 1983
Ozato, K. and Sachs, D. H.: Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2 bhaplotype reveal genetic control of isotype expression. J. Immunol. 126: 317–321, 1981
Ozato, K., Hansen, T. H., and Sachs, D.: Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex. J. Immunol. 125: 2473–2477, 1980
Ozato, K., Wan Y.-J., and Orrison, B. M.: Mouse major histocompatibility class I gene expression begins at midsomite stage and is inducible in earlier stage embryos by interferons. Proc. Natl. Acad. Sci. U.S.A. 82: 2427–2431, 1985
Shedlovsky, A., Clipson, L. J., Van de Berg, J. L., and Dove, W. F.: Strong teratocarcinoma transplantation loci, Gt-1 and Gt-2, flank H-2. Immunogenetics 13: 413–419, 1981
Tyan, M. L. and Cole, L. J.: Differential radiosensitivity of first and second-set responses to allogeneic and xenogeneic skin grafts in sublethally irradiated mice. Transplantation 1: 546–551, 1963
Uyttenhove, C., Maryanski, J., and Boon, T.: Escape of mouse mastocytoma P815 after nearly complete rejection is due to antigen-loss variants rather than immunosuppression. J. Exp. Med. 157: 1040–1052, 1983
Weissman, I. L.: Tumor immunity in vivo: Evidence that immune destruction of tumor leaves “bystander” cells intact. J. Natl. Cancer Inst. 51: 443–448, 1973
Wong, G. H. W., Bartlett, P. F., Clark-Lewis, I., Battye, F., and Schrader, J. W.: Inducible expression of H-2 and I-a antigens on brain cells. Nature 310: 688–691, 1984a
Wong, G. H. W., Clark-Lewis, I., Harris, A. W., and Schrader, J. W.: Effect of cloned interferon-y on expression of H-2 and Ia antigens on cell lines of hemopoietic, lymphoid, epithelial, fibroblastic and neuronal origin. Eur. J. Immunol. 14: 52–56, 1984b
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Moser, A.R., Johnson, L.L. & Dove, W.F. Mice coisogenically immunized against H-2 class I antigens on transfected l cells reject transplanted embryonal carcinoma cells. Immunogenetics 22, 533–541 (1985). https://doi.org/10.1007/BF00430301
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00430301