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Studies on polyol metabolism in Aspergillus niger

III. Purification and properties of sorbitol dehydrogenase

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Summary

Sorbitol dehydrogenase has been purified about 26 fold from a strain of Aspergillus niger, growing on sorbitol as the sole source of carbon. An absolute specificity of this enzyme for sorbitol, fructose, NAD and NADH was observed. The K m for sorbitol and fructose were found to be 9.8x10-5 M and 6.6x10-4 M respectively. The enzyme was inhibited by pCMB, NaF and other metal ions studied. The enzyme was slightly activated by Fe+++.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, M. S. University of Baroda, Baroda, India

    B. M. Desai, V. V. Modi & V. K. Shah

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  1. B. M. Desai
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  2. V. V. Modi
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  3. V. K. Shah
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Desai, B.M., Modi, V.V. & Shah, V.K. Studies on polyol metabolism in Aspergillus niger . Archiv. Mikrobiol. 67, 16–20 (1969). https://doi.org/10.1007/BF00413677

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  • DOI: https://doi.org/10.1007/BF00413677

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