Abstract
We recently described the production and characterization of an Ab-specific monoclonal antibody (mAb), designated K14.83-11, which specifically enhanced the alloreactivity of normal T lymphocytes, as well as highly selected Ab-specific T-cell lines and clones against the appropriate Ab molecule. In the present report, we have studied both the binding specificity and immunoenhancing activity of K14.83-11 in a number of genetic combinations to better characterize the possible modes through which this mAb achieves its T cell-enhancing activity. Results reported here (1) confirm and extend previous findings revealing the possible importance of the immunoglobulin G3 isotype of K14.83-11 in its immunoenhancing activity, and (2) show a distinction in the determinant binding and subsequent ability to elicit an enhancing effect. Surprisingly, K14.83-11 proved ineffective in enhancing autologous or Ab-restricted reactions. This system, then, may provide the means of comparing the functional importance of Ab expression in self-restricted and allogeneic reactions.
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Baxevanis, C. N., Wernet, D., Nagy, Z. A., Maurer, P. H., and Klein, J.: Genetic control of T-cell proliferative responses to poly(Glu40Alab60O) and poly(Glu51Lys34Lys15): Subregion specific inhibition of the responses with monoclonal la antibodies. Immunogenetics 11: 617–628, 1980
Davignon, D., Martz, E., Reynolds, T., Kurzinger, K., and Springer, T. A.: Monoclonal antibody to a novel lymphocyte functionassociated antigen (LFA-1). Mechanism of blockade of T lymphocyte-mediated killing and effects on other T and B lymphocyte functions. J. Immunol. 127: 590–595, 1981
Dorval, G., Welsh, K. I., and Wigzell: A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein A from Staphylococcus aureus. J. Immunol. Methods 7: 237–249, 1975
Ferguson, T. A., Beaman, K. D., and Iverson, G. M.: Isolation and characterization of a T suppressor factor by using a monoclonal antibody. J. Immunol. 134: 3163–3171, 1985
Haskins, K., Kubo, R., White, J., Pigeon, M., Kappler, T., and Marrack, P.: The mouse T cell receptor: Comparison of MHC restricted receptors on two T cell hybridomas. Cell 34: 727–737, 1983
Kimura, A. K. and Peck, A. B.: A novel anti-Ia monoclonal antibody which specifically enhances the corresponding T cell alloreactivity. J. Immunol. 133: 2360–2364, 1984
Klein, J., Figueroa, F., and David, C. S.: H-2 haplotypes, genes and antigens: Second listing. II. The H-2 complex. Immunogenetics 17: 553–596, 1983
Klotz, F. W., Gathings, W. E., and Cooper, M. D.: Development and distribution of B lineage cells in the domestic cat: Analysis with monoclonal antibodies to cat μ-, τ-, k- and λ-chains and heterologous anti-α antibodies. J. Immunol. 134: 95–100, 1985
Köhler, G., Fisher Lindahl, K., and Heusser, C.: Characterization of a monoclonal anti-H-2K b antibody. The immune system. In C. Steinberg and I. Lefkovits (eds.): Festschrift in Honor of Niels K. A. J. Jerne on the Occasion of his 70th Birthday, Vol. II, pt. 202–207, S. Karger, Basel, 1981
Kurnick, J. T., Osterg, L., Stegagno Kimura, A. K., Orn, A., and Sjoberg, O.: A rapid method for the separation of functional lymphoid cell populations of human and animal origin on PVPsilica (Percoll) density gradients. Scand. J. Immunol. 10: 563–573, 1979
Lerner, N. A., Mathis, L. A., Janeway, Jr., C. A., Jones, P. P., Schwartz, R. H., and Murphy, D. B.: Monoclonal antibody against an Ir gene product. J. Exp. Med. 152: 1085–1102, 1980
Longo, D. L. and Schwartz, R. H.: Inhibition of antigen-induced proliferation of T cells from radiation-induced bone marrow chimeras by a monoclonal antibody directed against an la determinant on the antigen presenting cell. Proc. Natl. Acad. Sci. U.S.A. 78: 514–518, 1981
McNicholas, J. M., Murphy, D. B., Mathis, L. A., Schwartz, R. H., Lerner, E. A., Janeway, Jr., C. A., and Jones, P.: Immune response gene function correlates with the expression of an Ia antigen. I. Preferential association of certain Ae and Eα chain results in a quantitative deficiency in expression of an Ae:Eα complex. J. Exp. Med. 155: 490–507, 1982
Möller, G. (ed).: Monoclonal antibodies against MHC products. Immunol. Rev. 47: 3–252, 1979
Nagy, Z., Baxevanis, C. N., Ishii, N., and Klein, J.: Ia antigens as restriction molecules in Ir gene-controlled T-cell proliferation. Immunol. Rev. 60: 59–83, 1981
Oi, T., Jones P. P., Goding, J. W., Herzenberg, L. A., and Herzenberg, L. A.: Properties of monoclonal antibodies to mouse Ig allotypes, H-2 and la antigens. Curr. Top. Microbiol. Immunol. 81: 115–132, 1978
Ozato, K., Mayer, N., and Sachs, D. H.: Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and la antigens. J. Immunol. 124: 533–540, 1980
Peck, A. B. and Bach, F. H.: A miniaturized mouse mixed leukocyte culture in mouse serum supplemented media. J. Immunol. Methods 3: 147–164, 1973
Peck, A. B., Klein, J., and Wigzell, H.: The mouse primed lymphocyte typing test. III. Dissociation of T lymphocyte-stimulating determinants and antibody-defined specificities of the I region-associated Ia antigens. J. Immunol. 125: 1078–1086, 1980
Pierres, A. and Pierres, M.: Fine specificity of a proliferating T cell clone activated by a conformational determinant of the I-Ek molecule. Immunogenetics 15: 399–412, 1982
Shaut, D. M., Wakeland, E. K., Maurer, P. H., and Peck, A. B.: Changes in the Ir gene controlled response phenotypes in mice of the AP and Ak family of alleles expressing naturally occurring variant molecules. J. Immunol. 133: 1410–1416, 1984
Shulman, M., Wilde, C. D., and Kohler, H.: A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269–270, 1978
Sprent, J.: Effects of blocking helper T cell induction in vivo with antila antibodies. Possible role of I-A/E hybrid molecules as restriction elements. J. Exp. Med. 152: 996–1010, 1980
Wassmer, P., Chan, C., Logdberg, L., and Shevach, E. M.: Role of the L3T4 antigen in T cell activation. II. Inhibition of T cell act tivation by monoclonal anti-L3T4 antibodies in the absence of accessary cells. J. Immunol. 135: 2237–2242, 1985
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Peck, A.B., Kimura, A.K. Characteristics of a T lymphocyte-enhancing Ab-specific monoclonal antibody. Immunogenetics 23, 284–291 (1986). https://doi.org/10.1007/BF00398790
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DOI: https://doi.org/10.1007/BF00398790