Abstract
The possible source of NADH, the energy donor for nitrate reductase (EC 1.6.6.1), has been studied using an in vivo assay involving freezing the material (leaves of Spinacea oleracea L.) in liquid nitrogen in order to render the tissue permeable to added substrates. Glycolysis and the pentose phosphate pathway were capable of generating NADH through glyceraldehyde-3-phosphate dehydrogenase. Malate and isocitrate were also capable of generating NADH white other organic acids tested were not, including glycolate which was ineffective even under anaerobic conditions.
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Cox, G.F., Davies, D.D.: Nicotinamide-adenine dinucleotide specific isocitrate dehydrogenase from pea mitochondria. Biochem. J. 105, 729–734 (1967)
Heber, U.: Metabolic exchange between chloroplasts and cytoplasm. Ann. Rev. Plant Physiol. 25, 393–421 (1974)
Hewitt, E.J.: Sand and water culture methods used in the study of plant nutrition. 2nd Ed. Commonwealth Bureau of Horticulture, Tech. Comm. 22, 1966
Hewitt, E.J.: Assimilatory nitrate-nitrite reduction. Ann. Rev. Plant Physiol. 26, 73–100 (1975)
Hewitt, E.J., Nicholas, D.J.D.: Enzymes of inorganic nitrogen metabolism. In: Modern methods of plant analysis, vol. 7, pp 67–172. Paech, K., Tracey, M.V. eds. Berlin-Göttingen-Heidelberg: Springer 1964
Jaworski, E.G.: Nitrate reductase assay in intact plant tissues. Biochem. Biophys. Res. Comm. 43, 1274–1279 (1971)
Jolly, S.O., Campbell, W., Tolbert, N.E.: NADPH and NADH-nitrate reductases from soybean leaves. Arch. Biochem. Biophys. 174, 431–439 (1976)
Klepper, L.A., Hageman, R.H.: Dependence of nitrate reduction on generation of reduced nicotinamide adenine dinucleotide by glycolytic metabolism of leaf tissue. Fed. Proc. Amer. Soc. Exp. Biol. 28, 467 (1969)
Klepper, L.A., Flesher, D., Hageman, R.H.: Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. Plant Physiol. 48, 580–590 (1971)
Lips, S.H.: Photorespiration and nitrate reduction. In Proc. 2nd Int. Congr. on Photosynthesis pp 2241–2249. Forti, G., Avron, M., Melandri, A. eds. The Hague: Junk 1971
Mulder, E.G., Boxma, R., Van Veen, W.L.: The effect of molybdenum and nitrogen deficiencies on nitrate reduction in plant tissues. Plant and Soil 10, 335–355 (1959)
Neyra, C.A., Hageman, R.H.: Relationships between carbon dioxide, malate, and nitrate accumulation and reduction in corn (Zea mays L.) seedlings. Plant Physiol. 58, 726–730 (1976)
Plaut, Z., Littan, A.: Interaction between photosynthetic CO2 fixation products and nitrate reduction in spinach and wheat leaves. In Proc. 3rd Int. Congr. on Photosynthesis, pp 1507–1516. Avron, M. ed. Amsterdam-London: Elsevier 1974
Rhodes, D., Stewart, G.R.: A procedure for in vivo determination of enzyme activity in higher plant tissue. Planta 118, 133–144 (1974)
Roth-Bejerano, N., Lips, S.H.: Glycolate dehydrogenase activity in higher plants. Israel J. Bot. 22, 1–7 (1973)
Tingey, D.T., Fites, R.C., Baharsjah, J.: Factors influencing nitrate reduction in soybean foliage. New Phytol. 73, 21–29 (1974)
Walker, D.A.: Chloroplast and cell: — The movement of certain key substances etc. across the chloroplast envelope. In MTP International Review Science, Biochemistry series one, vol. 11, Plant Biochemistry pp 1–49. Northcote D.H. ed. London: Butterworths 1974
Yamazaki, R.K., Tolbert, N.E.: Malate dehydrogenase in leaf peroxisomes. Biochim. Biophys. Acta 178, 11–20 (1969)
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Mann, A.F., Hucklesby, D.P. & Hewitt, E.J. Sources of reducing power for nitrate reduction in spinach leaves. Planta 140, 261–263 (1978). https://doi.org/10.1007/BF00390257
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DOI: https://doi.org/10.1007/BF00390257