Abstract
A system has been established from isolated intact chromoplasts of Narcissus pseudonarcissus flowers that synthesizes geranylgeraniol, an unknown polyprenoid alcohol, phytoene, and β-carotene from [1-14C]isopentenyl pyrophosphate in a good yeild. Long chain pyrophosphates are not accumulated. San 6706 inhibits the dehydrogenation of phytoene, whereas nicotine does not lead to an accumulation of lycopene. Separation and identification of polyprenoid lipids was performed by HPLC. The properties and advantages of the chromoplast system are discussed.
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Abbreviations
- IPP:
-
isopentenyl pyrophosphate
- TLC:
-
thin-layer chromatography
- HPLC:
-
high pressure liquid chromatography
- GC:
-
gas chromatography
- Sau 6706:
-
4-chloro-5-(dimethylamino)-2-α,α,α-(trifluoro-m-tolyl)3(2H)-pyridazinone
References
Bhat, P.V., de Luca, L.M., Wind, M.L. (1980) Reverse phase high-pressure liquid chromatographic separation of retinoids, including retinylphosphate and mannosylretinylphosphate. Anal. Biochem. 102, 243–248
Bickel, H., Schultz, G., (1976) Biosynthesis of plastoquinone and β-carotene in isolated chloroplasts. Phytochemistry 15, 1253–1255
Buggy, M.J., Britton, G., Goodwin, T.W. (1974) Terpenoid biosynthesis by chloroplasts isolated in organic solvents. Phytochemistry 13, 125–129
Charlton, J.M., Treharne, K.J., Goodwin, T.W. (1967) Incorporation of [2-14C]mevalonic acid into phytoene by isolated cloroplasts. Biochem. J. 105, 205–212
Hill, H.M., Rogers, L.J. (1974) Mevalonate activating enzymes and phosphatases in higher plants. Phytochemistry 13, 763–777
Islam, M.A., Lyrene, S.A., Miller, E.M., Porter, J.W. (1977) Dissociation of prelycopersene pyrophosphate synthetase from phytoene synthetase complex of tomato fruit plastids. J. Biol. Chem. 252, 1523–1525
Jungalwala, F.B., Porter, J.W. (1967) Biosynthesis of phytoene from isopentenyl and farnesyl pyrophosphates by a partially purified tomato enzyme system. Arch. Biochem. Biophys. 119, 209–219
Kleinig, H. (1975) On the utilization in vivo of lycopene and phytoene as precursors for the formation of carotenoid glucoside ester and on the regulation of carotenoid biosynthesis in Myxococcus fulvus. Eur. J. Biochem. 57, 301–308
Kleinig, H., Lempert, U. (1970) Phospholipid analysis on a micro scale. J. Chromatogr. 53, 595–597
Kleinig, H., Liedvogel, B. (1978) Fatty acid synthesis by isolated chromoplasts from the daffodil. [14C]Acetate incorporation and distribution of labelled acids. Eur. J. Biochem. 83, 499–505
Kushwaha, S.C., Suzue, G., Subbarayan, C., Porter, J.W. (1970) The conversion of phytone [14C]to acyclic, monocyclic, and dicyclic carotenes and the conversion of lycopene-15, 15-3H to mono- and dicyclic carotenes by soluble enzyme systems obtained from plastids of tomato fruits. J. Biol. Chem. 245, 4708–4717
Liedvogel, B., Kleinig, H. (1979) Galactolipid synthesis in chromoplasts in vitro. Specificities of acyl-and glactosyltransferases. Planta 144, 467–471
Liedvogel, B., Kleinig, H. (1980) Phosphate translocator and adenylate translocator in chromoplast membranes. Planta, 150, 170–173
Liedvogel, B., Sitte, P., Falk, H. (1976) Chromoplasts in the daffodil: fine structure and chemistry. Cytobiology 12, 155–174
Mann, A.F., Hucklesby, D.P., Hewitt, E.J. (1979) A modified Hanes and Isherwood spary for improved detection of phosphate esters in thin layer chromatography. Anal. Biochem. 96, 6
Maudinas, B., Bucholtz, M.L., Papastephanou, C., Katiyar, S.S., Briedis, A.V., Porter, J.W., (1977) The partial purification and properties of a phytoene synthesizing enzyme system. Arch. Biochem. Biophys. 180, 354–362
Moore, F.D., Shephard, D.C. (1977) Biosynthesis in isolated Acetabularia chloroplasts. II. Plastid pigments. Protoplasma 92, 167–175
Popják, G., Cornforth, J.W., Cornforth, R.H., Ryhage, R., Goodman, D.S. (1962) Studies on the biosynthesis of cholesterol. J. Biol. Chem. 237, 56–61
Porter, J.W., Spurgeon, S.L. (1979) Enzymatic synthesis of carotenes. Pure Appl. Chem. 51, 609–622
Ruzicka, L., Firmenich, G. (1939) Zur Kenntnis der Diterpene (37 Mitteilung). Synthese des aliphatischen Diterpenalkohols Geranyl-geraniol. Helv. Chim. Acta 22, 392–396
Schacterle, G.R., Pollack, R.L. (1973) A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal. Biochem. 51, 654–655
Schneider, M.M., Hampp, R., Ziegler, H. (1977) Envelope permeability to possible precursors of carotenoid biosynthesis during chloroplast-chromoplast transformation. Plant Physiol. 60, 518–520
Wellburn, A.R., Hampp, R. (1976) Uptake of mevalonate and acetate during plastid development. Biochem. J. 158, 231–233
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Beyer, P., Kreuz, K. & Kleinig, H. β-Carotene synthesis in isolated chromoplasts from Narcissus pseudonarcissus . Planta 150, 435–438 (1980). https://doi.org/10.1007/BF00390182
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DOI: https://doi.org/10.1007/BF00390182