Abstract
We have studied the ethanolic fermentation of D-xylose with Pachysolen tannophilus in batch cultures. We propose a model to predict variations in D-xylose consumed, and biomass and ethanol produced, in which we include parameters for the specific growth rate, for the consumption of D-xylose and production of ethanol either related or not to growth.
The ideal initial pH for ethanol production turned out to be 4.5. At this pH value the net specific growth rate was 0.26 h−1, biomass yield was 0.16 g.g−1, the cell-maintenance coefficient was 0.073 g.g−1.h−1, the parameter for ethanol production non-related to growth was 0.064 g.g−1,h−1 and the maximum ethanol yield was 0.32 g.g−1.
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Abbreviations
- A c :
-
Carbon atomic weight
- a d1/h:
-
Specific cell-maintenance rate defined in Eq. (8)
- c :
-
Mass fraction of carbon in the biomass
- E g/l:
-
Ethanol concentration
- f x :
-
Correction factor defined in Eq. (13)
- f′ x :
-
Correction factor defined in Eq. (13)
- f xi :
-
Correction factor defined in Eq. (14)
- k d1/h:
-
Death constant
- M E :
-
Ethanol molecular weight
- M s :
-
Xylose molecular weight
- M xi :
-
Xylitol molecular weight
- m g xylose/g biomass:
-
Maintenance coefficient for substrate
- m dg xylose/g biomass:
-
Maintenance coefficient when k d≠
- q Eg ethanol/g biomass.:
-
Specific ethanol production rate
- s g/l:
-
Residual xylose concentration
- s 0 g/l:
-
Initial xylose concentration
- t h :
-
Time
- x g/l:
-
Biomass concentration
- x 0 g/l:
-
Initial biomass concentration
- Y E/sg ethanol/g xylose:
-
Instantaneous ethanol yield
- ¯Y E/sg ethanol/g xylose:
-
Mean ethanol yield
- Y s/T E g ethanol/g xylose:
-
Theoretical ethanol yield
- Y s/* E g ethanol/g xylose:
-
Corrected instantaneous ethanol yield
- ¯Y s/* E g ethanol/g xylose:
-
Corrected mean ethanol yield
- Y x/sg biomass/g xylose:
-
Biomass yield
- ¯Y xi/sg xylitol/g xylose:
-
Mean xylitol yield
- α g ethanol/g biomass:
-
Growth-associated product formation parameter
- β g ethanol/g biomass.h:
-
Non-growth-associated product formation parameter
- β dg ethanol/g biomass.h:
-
Non-growth-associated product formation parameter when k d≠0
- Θ h:
-
Variable defined in Eq. (6) or Eq. (7)
- μ 1/h:
-
Specific growth rate
- μ m1/h:
-
Maximum specific growth rate
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Bravo, V., Camacho, F., Sánchez, S. et al. The effect of pH on kinetic and yield parameters during the ethanolic fermentation of D-xylose with Pachysolen tannophilus. Bioprocess Engineering 9, 159–165 (1993). https://doi.org/10.1007/BF00389924
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DOI: https://doi.org/10.1007/BF00389924