Abstract
Growth of autotrophically growing duck-weeds (Lemna gibba L., G1) was stimulated by sucrose. The rate of respiration increased when plants had been grown on sucrose (8.7 μmol O2 g-1 fresh weight (FW) h-1) and was reduced after growth without sucrose in the dark or under longday conditions (2.5 μmol O2 g-1 FW h-1). Photosynthesis was induced already by low light intensities (0.1 klx).
Short-time application of glucose or sucrose stimulated respiration in proportion to the hexose uptake rate. Sucrose is probably not taken up as the disaccharide. The transported sugar species after addition of sucrose are its hexose moieties produced by the high activity of the cell wall invertase. Fructose stimulated to a lesser extent; mannitol induced no enhancement; 2-deoxyglucose slightly inhibited O2 uptake. After mild carbon starvation of the plants the uptake of glucose and 3-O-methylglucose proceeded without any lag phase, with similar saturation kinetics in both cases. The initial uptake rate at substrate saturation was 2.6 μmol glucose g-1 FW h-1 in the dark. Light stimulated hexose uptake by 2 to 3 times. The results show that Lemna gibba has an energy-dependent constitutive system for hexose uptake.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- FW:
-
fresh weight
- LD:
-
long day
- SD:
-
short day
References
Cheeseman, J.M., Pickard, B.G.: Electrical characteristics of cells from leaves of Lycopersicon. Canad. J. Bot. 55, 497–510 (1977)
Decker, M., Tanner, W.: Respiratory increase and active hexose uptake of Chlorella vulgaris. Biochim. Biophys. Acta 266, 661–669 (1972)
Fekete, M.A.R., Vieweg, G.H.: Wandgebundene Disaccharidhydrolasen. Ber. Dt. Bot. Ges. 89, 313–319 (1976)
Fondy, B.R., Geiger, D.R.: Sugar selectivity and other characteristics of phloem loading in Beta vulgaris. Plant Physiol. 59, 953–966 (1977)
Giaquinta, R.: Phloem loading of sucrose. pH dependence and selectivity. Plant Physiol. 59, 750–755 (1977)
Higinbotham, N., Etherton, B., Foster, R.J.: Effect of external K, NH4, Na, Ca, Mg and H ions on the cell transmembrane electropotential of Avena coleoptile. Plant Physiol. 39, 196–203 (1964)
Hillman, W.S.: The Lemnaceae, or duckweeds. Bot. Rev. 27, 221–287 (1961)
Hutchings, V.M.: Sucrose and proton cotransport in Ricinus cotyledons. Ph. D. Thesis, Cambridge (1976)
Jeschke, W.D., Simonis, W.: Über die Wirkung von CO2 auf die lichtabhängige Cl--Aufnahme bei Elodea densa: Regulation zwischen nichtcyclischer und cyclischer Photophosphorylierung. Planta 88, 157–171 (1969)
Jones, M.G.K., Novacky, A., Dropkin, V.H.: Transmembrane potentials of parenchyma cells and nematode-induced transfer cells. Protoplasma 85, 15–37 (1975)
Kandeler, R., Hügel, B., Rottenburg, T.: Gegensätzliche Wirkung der Sprossalterung auf die Blütenbildung bei Lemna paucicostata und Lemna gibba. Biochem. Physiol. Pflanzen 165, 331–336 (1974)
Komor, E.: Proton-coupled hexose transport in Chlorella vulgaris. FEBS Lett. 38, 16–18 (1973)
Komor, E., Rotter, M., Tanner, W.: A proton-cotransport system in a higher plant: sucrose transport in Ricinus communis. Plant Sc. Lett. 9, 153–162 (1977)
Kriedemann, P., Beevers, H.: Sugar uptake and translocation in the castor bean seedling II. Sugar transformations during uptake. Plant Physiol. 42, 174–180 (1967)
Lüttge, U.: Nectar composition and membrane transport of sugars and amino acids: A review on the present state of nectar research. Apidologie 8 (1977) (in press)
Mitchell, P.: Translocations through natural membranes. Adv. Enzymol. 29, 33–87 (1967)
Novacky, A., Ullrich-Eberius, C.I., Lüttge, U.: Membrane potential changes during transport of hexoses in Lemna gibba G1. Planta 138, 263–270 (1978)
Pirson, A., Seidel, F.: Zell- und stoffwechselphysiologische Untersuchungen an der Wurzel von Lemna minor unter besonderer Berücksichtigung von Kalium- und Calciummangel. Planta 38, 431–473 (1950)
Pitman, M.G., Mertz, S.M., Graves, J.S., Pierce, W.S., Higinbotham, N.: Electrical potential differences in cells of barley roots and their relation to ion uptake. Plant Physiol. 47, 76–80 (1971)
Racusen, R.H., Galston, A.W.: Electrical evidence for rhythmic changes in the cotransport of sucrose and hydrogen ions in Samanea pulvini. Planta 135, 57–62 (1977)
Schuster, M.: Die Bedeutung von Starklicht und Kupfer für die phytochromgesteuerte Morphogenese von Lemna perpusilla. Thesis, Univ. Würzburg (1967)
Slayman, C.L., Slayman, C.W.: Depolarization of the plasma membrane of Neurospora during active transport of glucose: evidence for a proton-dependent cotransport system. Proc. Natl. Acad. Sci. USA 71, 1935–1939 (1974)
Slayman, C.L., Long, W.S., Lu, C.Y.-H.: The relationship between ATP and an electrogenic pump in the plasma membrane of Neurospora crassa. J. Membrane Biol. 14, 305–338 (1973)
Tanner, W., Grünes, R., Kandler, O.: Spezifität und Turnover des induzierbaren Hexose-Aufnahmesystems von Chlorella. Z. Pflanzenphysiol. 62, 376–386 (1970)
Ullrich-Eberius, C.I., Lüttge, U., Neher, L.: CO2 uptake by barley leaf slices as measured by photosynthetic O2 evolution. Z. Pflanzenphysiol. 79, 336–346 (1976a)
Ullrich-Eberius, C.I., Lüttge, U., Neher, L.: Energy relations of phosphate uptake and distribution in barley leaf slices as affected by cutting and adaptive ageing. Z. Pflanzenphysiol. 79, 347–359 (1976b)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ullrich-Eberius, C.I., Novacky, A. & Lüttge, U. Active hexose uptake in Lemna gibba G1. Planta 139, 149–153 (1978). https://doi.org/10.1007/BF00387140
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00387140