Abstract
Adenosine 5′-triphosphate-(ATP)-induced whole-cell currents were studied in human B-lymphocytes, transformed by the Epstein-Barr virus, by means of the tight-seal voltage-clamp technique. During bath application of ATP, the membrane conductance was increased. The change of membrane conductance occurred within milliseconds. The dose response relationship for the ATP4−-elicited membrane current (I p) was fitted by the Hill function with a Hill coefficient of 1 and a K D value of 0.2 mmol/l. Adenosine, as well as the Mg2+-bound form of ATP, did not effect the membrane conductance. I p did not desensitize within 1 min and could be evoked repeatedly up to 100 times in 1 cell in the presence of the G-protein blocker Guanosine 5′-o-(2-thiodiphosphate) (GDP [βS]). Therefore, it seems that ion channels in form of P 2Z purinoceptors are involved in the observed effects. The permeability (P) sequence for cations carrying I p was P Ca:P K:P Cs:P Na:P TRIS= 35∶2∶1.2∶1∶0.1. The reversal potential of I p was not changed by substitution of intracellular Cl− for aspartate, indicating that anions are not involved in the purinoceptor-dependent conductance. A single-channel conductance of P 2Z-receptor-dependent ion channels of about 3 pS was determined by noise analysis of I p.
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Bretschneider, F., Klapperstück, M., Löhn, M. et al. Nonselective cationic currents elicited by extracellular ATP in human B-lymphocytes. Pflügers Arch. 429, 691–698 (1995). https://doi.org/10.1007/BF00373990
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DOI: https://doi.org/10.1007/BF00373990