Abstract
We are mapping quantitative trait loci (QTLs) that influence ethanol-induced anesthesia (sleep time) in the Long-sleep (LS) and Short-sleep (SS) slected lines of mice. Fifty microsatellite-STS markers were initially screened for simple-sequence length polymorphisms between the LS and SS lines. Nineteen markers were polymorphic. Eleven markers unequivocally differentiated the LS and SS lines and were used to establish strain distribution patterns for the LSXSS series of recombinant inbred strains. Five markers each accounted for at least 5% of sleep-time genetic variance among the RI strains. Linkage of provisional QTLs detected among RIs will be confirmed or disproved in a large F2 population. This ongoing QTL-mapping project eventually will result in a strain distribution pattern for the LSXSS RI series with an average marker spacing of 5 centimorgans.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cornall, R.J., Aitman, T.J., Hearne, C.M., Todd, J.A. (1991). The generation of a library of PCR-analyzed microsatellite variants for genetic mapping of the mouse genome. Genomics 10, 874–881.
Crabbe, J.C., Young, E.R., Tam, B., Kosobud, A., Belknap, J.K., Laursen, S.E. (1986). Genetic differences in anticonvulsant sensitivity in mouse lines selectively bred for ethanol withdrawal severity. J. Pharmacol. Exp. Ther. 239, 154–159.
DeFries, J.C., Wilson, J.R., Erwin, V.G., Petersen, D.R. (1989). LSXSS recombinant inbred strains of mice: initial characterization. Alcohol Clin. Exp. Res. 13, 196–200.
Dietrich, W., Katz, H., Lincoln, S.E., Shin, H.-S., Friedman, J., Dracopoli, N., Lander, E.S. (1992). A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131, 423–447.
Dudek, B.C., Abbot, M.E. (1984). A biometrical genetic analysis of ethanol response in selectively bred long-sleep and short-sleep mice. Behav. Genet. 14, 1–19.
Erwin, V.G., Heston, W.D.W., McClearn, G.E., Deitrich, R.A. (1976). Effect of hypnotics on mice genetically selected for sensitivity to ethanol. Pharmacol. Biochem. Behav. 4, 679–683.
Haley, C.S., Knott, S.A. (1992). A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69, 315–324.
Hamada, H., Petrino, M.G., Takunaga, T. (1982). A novel repeated element with z-DNA-forming potential is widely found in evolutionary diverse eukaryotic genomes. Proc. Natl. Acad. Sci. USA 79, 6465–6469.
Hearne, C.M., McAleer, M.A., Love, J.M., Aitman, T.J., Cornall, R.J., Ghosh, S., Knight, A.M., Prins, J.-B., Todd, J.A. (1991). Additional microsatellite markers for mouse genome mapping. Mammalian Genome 1, 273–282.
Heston, W.D.W., Erwin, V.G., Anderson, S.M., Robbins, H. (1974). A comparison of the effects of alcohol in mice selectively bred for differences in ethanol sleep-time. Life Sci 14, 365–370.
Howerton, T.C., O'Connor, M.F., Collins, A.C. (1983). Differential effects of long-chain alcohols in long- and short-sleep mice. Psychopharmacology 79, 313–317.
Johnson, T.E., DeFries, J.C., Markel, P.D. (1992). Mapping quantitative trait loci for behavioral traits in the mouse. Behav. Genet. 22, 635–653.
Li, T.-K., Lumeng, L., McBride, W.J., Waller, M.B. (1981). Indiana selection studies on alcohol-related behaviors. In Development of Animal Models as Pharmacogenetic Tools, G.E. McClearn, R.A. Deitrich, V.G. Erwin, eds. (Washington, D.C.: U.S. Government Printing Office DHHS Publication No. ADM 81-1133), pp. 171–191.
Litt, M., Luty, J.A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Hum. Genet. 44, 397–401.
Love, J.M., Knight, A.M., McAleer, M.A., Todd, J.A. (1990). Towards construction of a high resolution map of the mouse genome using PCR-analyzed microsatellites. Nucleic Acids Res. 18, 4123–4130.
Manly, K.F. (1993). A Macintosh program for storage and analysis of experimental genetic mapping data. Mammalian Genome 4, 303–313.
Manly, K.F., Elliott, R.W. (1991). RI Manager, a microcomputer program for analysis of data from recombinant inbred strains. Mammalian Genome 1, 123–126.
McClearn, G.E., Kakihana, R. (1981). Selective breeding for ethanol sensitivity: short-sleep and long-sleep mice. In Development of Animal Models as Pharmacogenetic Tools, G.E. McClearn, R.A. Deitrich, V.G. Erwin, eds. (Washington, D.C.: U.S. Govenment Printing Office DHHS Publication No. ADM 81-1133), pp. 147–159.
Olson, M., Hood, L., Cantor, C., Botstein, D. (1989). A common language for physical mapping of the human genome. Science 245, 1434–1435.
Plomin, R., McClearn, G.E., Gora-Maslak, G., Neiderhiser, J.M. (1991). Use of recombinant inbred strains to detect quantitative trait loci associated with behavior. Behav. Genet. 21, 99–116.
Sorenson, S., Dunwiddie, T., McClearn, G.E., Freedman, R., Hoffer, B. (1981). Ethanol-induced depression in cerebellar and hippocampal neurons of mice selectively bred for differences in ethanol sensitivity: an electrophysiological study. Pharmacol. Biochem. Behav. 14, 227–234.
Strauss, W.M. (1989). Preparation of genomic DNA from mammalian tissue. In Current Protocols in Molecular Biology, F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl, eds. (New York: Greene Publishing Associates and Wiley-Interscience) 1, 2.2.1–2.2.3.
Weber, J.L., May, P.E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Hum. Genet. 44, 388–396.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Markel, P.D., Johnson, T.E. Initial characterization of STS markers in the LSXSS series of recombinant inbred strains. Mammalian Genome 5, 199–202 (1994). https://doi.org/10.1007/BF00360545
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00360545