Abstract
We have cloned the his7 + gene of the fission yeast Schizosaccharomyces pombe by complementation of the recessive mutant allele his7-366. The his7 + gene is able to complement a mutation of the Escherichia coli hisI gene, suggesting that his7 + encodes a phosphoribosyl-AMP cyclohydrase. Subcloning experiments localize the gene to a 1.9-kb XbaI-BglII fragment. We describe the construction of plasmids to facilitate the use of his7 + as a selectable marker in S. pombe studies. Plasmid pEA2 carries his7 + cloned into the pUC18 polylinker. From either pEA2 or the original his7 + clone, pMN1, fragments carrying his7 + can be isolated using a variety of restriction enzymes for the construction of gene disruptions. Plasmid pEA500 is a cloning vector that carries his7 + and ars1, yet retains the ability to use the blue/white color screen to identify recombinants.
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Communicated by R.J. Rothstein
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Apolinario, E., Nocero, M., Jin, M. et al. Cloning and manipulation of the Schizosaccharomyces pombe his7 +gene as a new selectable marker for molecular genetic studies. Curr Genet 24, 491–495 (1993). https://doi.org/10.1007/BF00351711
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DOI: https://doi.org/10.1007/BF00351711