Skip to main content
SpringerLink
Log in

Conjugal transfer of nopaline and agropine Ti-plasmids —The role of agrocinopines

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The conjugative behaviour of nopaline and agropine Ti-plasmids has been investigated. Using a technique which avoids enrichment of transconjugants on a mating medium we have shown that preculture in the presence of agrocinopines A or B of donor strains harbouring nopaline Ti plasmids promotes plasmid transfer whereas preculture of the same strains in the presence of nopaline has no such effect. Similarly, preculture in the presence of agrocinopines C or D promotes Ti-plasmid transfer from strains harbouring agropine Ti-plasmids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Albinger G, Beiderbeck R (1977) Übertragung der Fähigkeit zur Wurzelinduction von Agrobacterium rhizogenes auf A. tumefaciens. Phytopathol Z 90:306–310

    Google Scholar 

  • Chilton M-D, Drummond HJ, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11:263–271

    Google Scholar 

  • Cooper D, Firmin JL (1977) Synthesis of nopaline (N2−1,3 dicarboxypropyl) arginine and isonopaline. Org Prep Proced Int 9:99–101

    Google Scholar 

  • Depicker A, Van Montagu M, Schell J (1978) Homologous DNA sequences in different Ti-plasmids are essential for oncogenicity. Nature (London) 275:250–253

    Google Scholar 

  • Ellis JG, Kerr A, Van Montagu M, Schell J (1979a) Agrobacterium: genetic studies on agrocin 84 production and the biological control of crown gall. Physiol Plant Pathol 15:311–319

    Google Scholar 

  • Ellis JG, Kerr A, Tempé J, Petit A (1979b) Arginine catabolism: a new function of both octopine and nopaline Ti-plasmids of Agrobacterium. Mol Gen Genet 173:263–269

    Google Scholar 

  • Ellis JG, Murphy PJ (1981) Four new opines from crown gall tumours — their detection and properties. Mol Gen Genet 181:36–43

    Google Scholar 

  • Firmin JL, Fenwick RG (1977) N2(1–3 dicarboxypropyl) ornithine in crown gall tumours. Phytochemistry 16:761–762

    Google Scholar 

  • Firmin JL, Fenwick GR (1978) Agropine — a major new plasmiddetermined metabolite in crown gall tumours. Nature (London) 276:842–844

    Google Scholar 

  • Goldmann A, Thomas DW, Morel G (1969) Sur la structure de la nopaline métabolite anormale de certaines tumeurs de crowngall. CR Acad Sci (Paris) 268:852–854

    Google Scholar 

  • Guyon P, Chilton M-D, Petit A, Tempé J (1980) Agropine in “null type” crown gall tumors; evidence for the generality of the opine concept. Proc Natl Acad Sci USA 77:269302697

    Google Scholar 

  • Holsters M, Silva B, Van Vliet F, Genetello C, De Block M, Dhaese P, Depicker A, Inzé D, Engler G, Villarroel R, Van Montagu M, Schell J (1980) The functional organization of the nopaline A. tumefaciens plasmid pTiC58. Plasmid 3:212–230

    Google Scholar 

  • Hooykaas PJJ (1979) Ph. D. thesis, Rijksuniversiteit Leiden

  • Keane PJ, Kerr A, New PB (1970) Crown gall of stone fruit. II. Identification and nomenclature of Agrobacterium isolates. Austr J Biol Sci 23:585–595

    Google Scholar 

  • Kemp JD (1977) A new amino-acid derivative present in crown gall tumor tissue. Biochem Biophys Res Commun 74:862–868

    Google Scholar 

  • Kerr A (1969) Transfer of virulence between isolates of Agrobacterium. Nature (London) 223:1175–1176

    Google Scholar 

  • Kerr A, Htay K (1974) Biological control of crown gall through bacteriocin production. Physiol Plant Pathol 4:37–44

    Google Scholar 

  • Kerr A, Manigault P, Tempé J (1977) Transfer of virulence in vivo and in vitro in Abrobacterium. Nature (London) 265:560–561

    Google Scholar 

  • Kerr A, Roberts WP (1976) Agrobacterium: Correlation between and transfer of pathogenicity, octopine and nopaline metabolism and bacteriocin 84 sensitivity. Physiol Plant Pathol 9:205–211

    Google Scholar 

  • Klapwijk PM, Schilperoort RA (1979) Negative control of octopine degradation and transfer genes of octopine Ti-plasmids in Agrobacterium tumefaciens. J Bacteriol 139:424–431

    Google Scholar 

  • Klapwijk PM, Scheuldermon T, Schilperoort RA (1978) Co-ordinated regulation of octopine degradation and conjugative transfer of Ti-plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons. J Bacteriol 136:775–785

    Google Scholar 

  • Lemmers M, De Beuckeleer M, Holsters M, Zambryski P, Depicker A, Hernalsteens JP, Van Montagu M, Schell J (1980) Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline crown gall tumors. J Mol Biol 144:355–378

    Google Scholar 

  • Lioret C (1957) Les acides aminés libres des tissus de crown gall cultivés in vitro. CR Acad Sci (Paris) 244:2171–2174

    Google Scholar 

  • Ménagé A, Morel G (1964) Sur la présence d'octopine dans les tissus de crown gall. CR Acad Sci (Paris) 259:2795–4796

    Google Scholar 

  • Ménagé A, Morel G (1965) Sur la présence d'un acide aminé nouveau dans le tissu de crown-gall. CR Soc Biol (Paris) 159:561–562

    Google Scholar 

  • Moore L, Warren G, Strobel G (1979) Involvement of a plasmid in the hairy root disease of plants caused by Agrobacterium rhizogenes. Plasmid 2:617–626

    Google Scholar 

  • Murphy PJ, Roberts WP (1979) A basic for agrocin 84 sensitivity in Agrobacterium. J Gen Microbiol 114:207–213

    Google Scholar 

  • Petit A, Dessaux Y, Tempé J (1978a) The biological significance of opines. I. A study of opine catabolism by Agrobacterium tumefaciens. Proc. IVth Int Conf Plant Pathogenic Bacteria, Angers, I.N.R.A., 143–152

  • Petit A, Tempé J (1978) Isolation of Agrobacterium Ti-plasmid regulatory mutants. Mol Gen Genet 167:147–155

    Google Scholar 

  • Petit A, Tempé J, Kerr A, Hosters M, Van Montagu M, Schell J (1978b) Substrate induction of conjugative activity of Agrobacterium tumefaciens. Nature (London) 271:570–572

    Google Scholar 

  • Schell J, Van Montagu M, De Beuckeleer M, De Block M, Depicker A, De Wilde M, Engler G, Genetello C, Hernalsteens JP, Holsters M, Seurinck J, Silva B, Van Vliet F, Villarroel R (1979) Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid an the plant host. Proc R Soc Lond B 204:251–266

    Google Scholar 

  • Tate ME, Ellis JG, Kerr A, Tempé J, Murray KE, Shaw KJ (1982) Agropine: a revised structure. Carbohydr Res (in press)

  • Tempé J, Estrade C, Petit A (1978) The biological significance of opines. II. The conjugative activity of the Ti-plasmids of Agrobacterium tumefaciens. Proc IVth Conf Plant Pathogenic Bacteria, Angers, I.N.R.A. 153–160

  • Tempé J, Guyon P, Petit A, Ellis JG, Tate ME, Kerr A (1980) Préparation et propriétés de nouveaux substrats cataboliques pour deux types de plasmides oncogènes d'Agrobacterium tumefaciens. CR Acad Sci Paris, Série D 290:1173–1176

    Google Scholar 

  • Van Larebeke N, Engler G, Holsters M, Van den Elsacker S, Zaenen I, Schilperoort RA, Schell J (1974) Large plasmid in Agrobacterium tumefaciens essential for crown gall inducing ability. Nature (London) 252:169–170

    Google Scholar 

  • Van Larebeke N, Genetello C, Schell J, Schilperoort RA, Hermans AK, Hernalsteens JP, Van Montagu M (1975) Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature (London) 255:742–743

    Google Scholar 

  • Watson B, Currier TC, Gordon MP, Chilton M-D, Nester EW (1975) Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol 123:255–264

    Google Scholar 

  • White FF, Nester EW (1980) Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes. J Bacteriol 141:1134–1141

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Pathology, Waite Agricultural Research Institute, 5064, Glen Osmond, South Australia

    J. G. Ellis & A. Kerr

  2. Laboratoire de Recherches sur le Crown-Gall Station de Génétique et d'Amélioration de Plantes, CNRA, F-78000, Versailles, France

    A. Petit & J. Tempe

Authors
  1. J. G. Ellis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Kerr
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Petit
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Tempe
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J. Schell

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ellis, J.G., Kerr, A., Petit, A. et al. Conjugal transfer of nopaline and agropine Ti-plasmids —The role of agrocinopines. Mol Gen Genet 186, 269–274 (1982). https://doi.org/10.1007/BF00331861

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00331861

Keywords

Search

Navigation