Summary
We have developed a rapid, simple transformation procedure for intact cells of Hansenula polymorpha. It is a modification of the LiAc method and can yield 104–105 transformants/μg DNA. The use of stationaryphase cells, a high cell density per plate and a hear pulse at 50°C for 10 min are among major modifications of the original method. We have also found that, within the XhoI-SalI chromosomal fragment of Saccharomyces cerevisae containing the LEU2 gene, a sequence exists which supports autonomous replication of plasmid moleculles in H. polymorpha.
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Berardi, E., Thomas, D.Y. An effective transformation method for Hansenula polymorpha . Curr Genet 18, 169–170 (1990). https://doi.org/10.1007/BF00312606
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DOI: https://doi.org/10.1007/BF00312606