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Quinidine-sensitive K+ channels in the basolateral membrane of embryonic coprodeum epithelium: regulation by aldosterone and thyroxine

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Abstract

Basolateral K+ channels and their regulation during aldosterone- and thyroxine-stimulated Na+ transport were studied in the lower intestinal epithelium (coprodeum) of embryonic chicken in vitro. Isolated tissues of the coprodeum were mounted in Ussing chambers and investigated under voltage-clamped conditions. Simultaneous stimulation with aldosterone (1 μmol·l-1) and thyroxine (1 μmol·l-1) raised short-circuit current after a 1- to 2-h latent period. Maximal values were reached after 6–7 h of hormonal treatment, at which time transepithelial Na+ absorption was more than tripled (77±11 μA·cm-2) compared to control (24±8 μA·cm-2). K+ currents across the basolateral membrane with the pore-forming antibiotic amphotericin B and application of a mucosal-to-serosal K+ gradient. This K+ current could be dose dependently depressed by the K+ channel blocker quinidine. Fluctuation analysis of the short-circuit current revealed a spontaneous and a blocker-induced Lorentzian noise component in the power density spectra. The Lorentzian corner frequencies increased linearly with the applied blocker concentration. This enabled the calculation of single K+ channel current and K+ channel density. Single K+ channel current was not affected by stimulation, whereas the number of quinidine-sensitive K+ channels in the basolateral membrane increased from 11 to 26·106·cm-2 in parallel to the hormonal stimulation transepithelial Na+ transport. This suggests that the basolateral membrane is a physiological target during synergistic aldosterone and thyroxine regulation of transepithelial Na+ transport for maintaining intracellular K+ homeostasis.

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Abbreviations

f :

frequency

f c :

Lorentzian corner frequency

g K :

single K+ channel conductance

HEPES:

N-2-hydroxyethylpiperazin-N'-2-ethansulfonic acid

i K :

single K+ channel current

IAmpho :

amphotericin B induced K+ current

I sc :

short-circuit current

I K :

quinidine blockable K+ current

I max :

maximally blocked current by quinidine

IC 50 :

half-maximal blocker concentration

k on, k off :

on- and off-rate coefficients of reversible single channel block by quinidine

M K :

number of conducting K+ channels

[Q]:

quinidine concentration

R t :

transepithelial resistance

S :

spectral density

S o :

Lorentzian plateau

TBM cells:

toad urinary bladder cell line

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Authors and Affiliations

  1. Institut für Tierphysiologie, Justus-Liebig-Universität, Wartweg 95, D-35392, Giessen, Germany

    B. Illek, H. Fischer & W. Clauss

Authors
  1. B. Illek
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  2. H. Fischer
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  3. W. Clauss
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Present address: University of California at Berkeley, Dept. of Molecular and Cell Biology Berkeley, CA 94720, USA

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Illek, B., Fischer, H. & Clauss, W. Quinidine-sensitive K+ channels in the basolateral membrane of embryonic coprodeum epithelium: regulation by aldosterone and thyroxine. J Comp Physiol B 163, 556–562 (1993). https://doi.org/10.1007/BF00302114

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