Abstract
The DNA metabolism of mouse spermatogenic cells was investigated by intravenous administration of isotope and Staput fractionation of the cells. The pattern of metabolism is virtually identical with that observed in Lilium microsporocytes. A programmed single strand nicking of DNA occurs at pachytene such that at least 50% of the DNA is in the form of 62S fragments. Repair replication of endogenously nicked sites is fully achieved during pachytene. The sites of nicking and repair are preferentially located in sequences that are repeated about 400 times. These results are considered as strong evidence for a universal pattern of meiotic prophase DNA metabolism which is associated with crossing-over.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allen, J.W., Latt, S.A.: In vivo BrdU-33258 Hoechst analysis of DNA replication kinetics and sister chromatid exchange formation in mouse somatic and meiotic cells. Chromosoma (Berl.) 58, 325–340 (1976)
Britten, R.J.: DNA sequence interspersion and a speculation about evolution. In: Evolution of genetic systems (H.H. Smith, ed.), pp. 80–94. New York: Gordon and Breach 1972
Britten, R.J., Kohne, D.E.: Repeated sequences in DNA. Science 161, 529–540 (1968)
Chandley, A.C., Hotta, Y., Stern, H.: Biochemical analysis of meiosis in the male mouse I. Separation and DNA labelling of specific spermatogenic stages. Chromosoma (Berl.) 62, 243–253 (1977)
Hotta, Y., Stern, H.: DNA scission and repair during pachytene in Lilium. Chromosoma (Berl.) 46, 279–296 (1974)
Jacobson, G.K., Pinon, R., Esposito, R.E., Esposito, M.S.: Single strand scissions of chromosomal DNA during commitment to recombination at meiosis. Proc. nat. Acad. Sci. (Wash.) 72, 1887–1891 (1975)
Kofman-Alfaro, S., Chandley, A.C.: Radiation initiated DNA synthesis in spermatogenic cells of the mouse. Exp. Cell Res. 69, 33–44 (1971)
Meistrich, M.L., Reid, B.O., Barcellona, W.J.: Meiotic DNA synthesis during mouse spermatogenesis. J. Cell Biol. 64, 211–222 (1975)
Ormerod, M.G.: Radiation-induced strand breaks in the DNA of mammalian cells. In: Biology of radiation carcinogenesis (J.M. Yuhas, R.W. Tennant and J.D. Regan, eds.), pp. 67–92. New York: Raven Press 1976
Painter, R.B., Cleaver, J.E.: Repair replication in HeLa cells after large doses of x-irradiation. Nature (Lond.) 216, 369–370 (1967)
Stern, H.: Isolation and purification of plant nucleic acids from whole tissues and from isolated nuclei. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), Vol. XII B, pp. 100–112. New York: Academic Press Inc. 1968
Stern, H., Hotta, Y.: Biochemical controls of meiosis. Ann. Rev. Genet. 7, 37–66 (1974)
Stern, H., Hotta, Y.: Biochemistry of meiosis. Phil. Trans. roy. Soc. (Lond.) B277, 277–293 (1977)
Studier, F.W.: Sedimentation studies of the size and shape of DNA. J. molec. Biol. 11, 373–390 (1965)
Zimm, B.H., Schumaker, V.N.: Anomalies in sedimentation. V. Chains at high fields, practical consequences. Biophys. Chem. 5, 265–270 (1976)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hotta, Y., Chandley, A.C. & Stern, H. Biochemical analysis of meiosis in the male mouse. Chromosoma 62, 255–268 (1977). https://doi.org/10.1007/BF00286047
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00286047