Summary
Glomerular basement membrane synthesis was measured in vivo in diabetic rats at various times after streptozotocin injection. Simultaneously the type IV collagen concentration in serum was determined by a radioimmunoassay specific for the terminal cross-linking domain, 7S collagen. Basement membrane collagen synthesis and the 7S collagen levels were markedly raised in diabetic animals 18–34 days after streptozotocin injection and showed a significant correlation. The results suggest that the amount of serum 7S collagen reflects increased basement membrane synthesis in a diabetic situation. Both basement membrane collagen synthesis and serum 7S collagen concentration also show a positive correlation with the blood glucose levels of diabetic rats. This indicates that the degree of metabolic dysregulation influences basement membrane synthesis.
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Hasslacher, C., Reichenbacher, R., Gechter, F. et al. Glomerular basement membrane synthesis and serum concentration of type IV collagen in streptozotocin-diabetic rats. Diabetologia 26, 150–154 (1984). https://doi.org/10.1007/BF00281124
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DOI: https://doi.org/10.1007/BF00281124