Summary
The rplY gene of Escherichia coli K12 encoding ribosomal protein L25 was cloned from the ordered clone bank and located at coordinate 2,291 kb on the physical map of E. coli. Determination of the nucleotide sequence indicated that the coding region contains 285 nucleotide pairs including a translational initiator and terminator. The amino acid sequence of the protein deduced from the nucleotide sequence matched completely the sequence determined for ribosomal protein L25. The coding region was found to be preceded by a typical promoter-like sequence and was followed by a DNA region capable of forming a secondary structure characteristic of a transcriptional terminator. Thus, the gene was concluded to constitute a transcriptional unit (operon). A preliminary analysis by Northern blot supported this conclusion. The codnn usage pattern of the rplY gene is characteristic of the ribosomal protein genes in E. coli.
References
Bachmann B (1990) Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev 54:130–197
Bitar KG, Wittmann-Liebold B (1975) The primary structure of the 5S rRNA binding protein L25 of Escherichia coli ribosome. Hoppe-Seyler's Z Physiol Chem 356:1343–1352
Dovgas NU, Markova LF, Mednikova TA, Vinokurov LM, Alakov YB, Ovchinnikov YA (1975) The primary structure of the 5S RNA binding protein L25 from Escherichia coli ribosomes. FEBS Lett 53:351–354
Fearon K, Mason TL (1988) Structure and regulation of a nuclear gene in Saccharomyces cerevisiae that specifies MRP7, a protein of the large subunit of the mitochondrial ribosome. Mol Cell Biol 8:3636–3646
Grohmann L, Graack H-R, Kitakawa M (1989) Molecular cloning of the nuclear gene for mitochondrial ribosomal protein YmL31 from Saccharomyces cerevisial
Isono K (1984) A computer program package for storing and retrieving DNA/RNA and protein sequence data. Nucleic Acids Res 12:101–112
Isono S, Thamm S, Kitakawa M, Isono K (1985) Cloning and nucleotide sequencing of the genes for ribosomal proteins S9 (rpsl) and L13 (rpIM) of Escherichia coli. Mol Gen Genet 198:279–282
Kang W-K, Matsushita Y, Isono K (1990) Cloning and analysis of YMR26, the nuclear gene for a mitochondrial ribosomal protein in Saccharomyces cerevisiae. Mol Gen Genet (in press)
Kitakawa M, Isono K, Grohmann L, Graack H-R (1990) Cloning and characterization of nuclear genes for two mitochondrial ribosomal proteins in Saccharomyces cerevisiae. Nucleic Acids Res 18:1521–1529
Kohara Y, Akiyama K, Isono K (1987) The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508
Lindahl L, Zengyel JM (1986) Ribosomal genes in Escherichia coli. Annu Rev Genet 20:297–326
Matsushita Y, Kitakawa M, Isono K (1989) Cloning and analysis of the nuclear genes for two mitochondrial ribosomal genes in yeast. Mol Gen Genet 219:119–124
Mizusawa S, Nishimura S, Seela F (1986) Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res 14:1319–1324
Myers AM, Crivellone MD, Tzagoloff A (1987) Assembly of the mitochondrial membrane system: MRPI and MRP2, two yeast nuclear genes coding for mitochondrial ribosomal proteins. J Biol Chem 262:3388–3397
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature 322:572–574
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schnier J, Isono K (1979) The gene for ribosomal protein L25 (rpf) maps at 47.3 min near alA in Escherichia coli. Mol Gen Genet 176:313–318
Schnier J, Isono K (1982) The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1. Nucleic Acids Res 10:1857–1865
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) The complete nucleotide sequence of tobacco chloroplast genome. EMBO J 5:2043–2049
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Subramanian AR (1975) Copies of proteins L7 and L12 and heterogeneity of the large subunit of Escherichia coli ribosome. J Mol Biol 95:1–8
Tanaka Y, Tsujimura A, Fujita N, Isono S, Isono K (1989) Cloning and analysis of an Escherichia coli operon containing the rpmF, gene for ribosomal protein L32 and the gene for a 30 kDa protein. J Bacteriol 171:5707–5712
Author information
Authors and Affiliations
Additional information
Communicated by J. Lengeler
Rights and permissions
About this article
Cite this article
Uemura, Y., Isono, S. & Isono, K. Cloning, characterization, and physical location of the rplY gene which encodes ribosomal protein L25 in Escherichia coli K12. Mol Gen Genet 226, 341–344 (1991). https://doi.org/10.1007/BF00273625
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00273625