Summary
The P type conjugative plasmid RP4 has been shown to be able to promote the transfer of the Agrobacterium Ti-plasmid. The results provide additional evidence that agrocin 84 sensitivity, exclusion of phage AP1, ability to catabolize the guanidine derivatives octopine and nopaline and tumor inducing ability, are Ti-plasmid determined properties. Furthermore, the results strongly support the notion that at least part of the Ti-plasmid is transferred from the bacterium to the target plant cells, since it was demonstrated that Ti-plasmid linked genes specify the synthesis of octopine or nopaline by crown-gall tumor cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bomhoff, G., Klapwijk, P.M., Kester, C.M., Schilperoort, R., Hernalsteens, J.P., Schell, J.: Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens. Molec. gen. Genet. 145, 177–181 (1976)
Chilton, M.D., Ferrand, S.K., Levin, R., Nester, E.W.: RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence. Genetics 83, 609–618 (1976)
Datta, N., Hedges, R.W., Shaw, E.J., Sykes, R.B., Richmond, M.H.: Properties of an R factor from Pseudomonas aeruginosa. J. Bact. 108, 1244–1248 (1971)
Engler, G., Holsters, M., Van Montagu, M., Schell, J., Hernalsteens, J.P., Schilperoort, R.A.: Agrocin 84 sensitivity: A plasmid determined property in Agrobacterium tumefaciens. Molec. gen. Genet. 138, 345–349 (1975)
Gordon, M.P., Ferrand, S.K., Sciaky, D., Montoya, A., Chilton, M.D., Merlo, D., Nester, E.W.: The crown-gall problem. In: Molecular biology of plants. Symposium University of Minnesota, St.-Paul, Editor I. Rubinstein (1976)
Hooykaas, P.J.J., Klapwijk, P.M., Nuti, M.P., Schilperoort, R.A.: Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent agrobacteria and to Rhizobium ex planta. Submitted to J. gen. Microbiol.
Kerr, A.: Transfer of virulence between strains of Agrobacterium. Nature (Lond.) 223, 1175–1176 (1969)
Kerr, A.: Acquisition of virulence by non pathogenic isolates of Agrobacterium radiobacter. Physiol. Pl. Path. I, 241–246 (1971)
Manigault, P., Kurkdjian, A.: Influence de la lumière sur le développement des tumeurs de la plantule de pois, Pisum Sativum L., induites par Agrobacterium tumefaciens (Smith et Town) conn. C.R. Acad. Sci. (Paris) Sér. D 264, 2304–2306 (1967)
Messens, E., Van Montagu, M., Schell, J.: Synthesis of D-Nopaline, an unusual amino-acid produced in crown-gall tumors. Manuscript in preparation (1977)
Olsen, R.H., Siak, J., Gray, R.H.: Characteristics of PRDI a plasmid-dependent-broad host-range DNA bacteriophage. J. Virol. 14, 689–699 (1974)
Radloff, R., Bauer, W., Vinograd, J.: A dye buoyant density method for the detection and isolation of closed circular duplex DNA. PNAS 57, (1514), Nr. 5 (1967)
Roberts, R.J.: Restriction endonucleases. C.R.C. Crit. Rev. Biochem. 4, 123–164 (1976)
Sharp, P.A., Sugden, B., Sambrook, J.: Detection of two restriction endonuclease activities in Hemophilus parainfluenzae using analytical agarose ethidium bromide electrophoresis. Biochemistry 12, 3055–3063 (1973)
Schell, J., Van Montagu, M., De Picker, A., De Waele, D., Engler, G., Genetello, Ch., Hernalsteens, J.P., Holsters, M., Silva, B., Van den Elsacker, S., Van Larebeke, N., Zaenen, I.: Crowngall: Bacterial plasmids as oncogenic elements for eucaryotic cells. In: Molecular biology of plants. Symposium University of Minnesota, St.-Paul, Editor I. Rubinstein (1976)
Stanisich, V.A.: The properties and host-range of male specific bacteriophages of Pseudomonas aeruginosa. J. gen. Microbiol. 84, 332–342 (1974)
Sugden, B., De Troy, B., Roberts, R.J., Sambrook, J.: Agarose slabgel electrophoresis equipment. Anal. Biochem. 68, Nr. 1, 36 (1975)
Van Larebeke, N., Engler, G., Holsters, M., Van den Elsacker, S., Zaenen, I., Schilperoort, R.A., Schell, J.: Large plasmid in Agrobacterium tumefaciens essential for crown-gall inducing ability. Nature (Lond.) 252, 169–179 (1974)
Van Larebeke, N., Genetello, Ch., Schell, J., Schilperoort, R.A., Hermans, A.K., Hernalsteens, J.P., Van Montagu, M.: Acquisition of tumour inducing ability by non-oncogenic Agrobacteria as a result of plasmid transfer. Nature (Lond.) 225, 742–743 (1975)
Vervliet, G., Holsters, M., Teuchy, H., Van Montagu, M., Schell, J.: Characterization of different plaque-forming and defective temperature phages in Agrobacterium strains. J. gen. Virol. 26, 33–48 (1974)
Watson, B., Currier, T.C., Gordon, M.P., Chilton, M.D., Nester, E.W.: Plasmid required for virulence of Agrobacterium tumefaciens. J. Bact. 123, 255–264 (1975)
Yamada, S., Itano, H.A.: Phenanthrenequinone as an analytical reagent for arginine and other mono-substituted guanidines. Biochim. Biophys. 130, 538–540 (1966)
Zaenen, I., Van Larebeke, N., Teuchy, H., Van Montagu, M., Schell, J.: Supercoiled circular DNA in crown-gall inducing Agrobacterium strains. J. molec. Biol. 86, 109–127 (1974)
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Van Larebeke, N., Genetello, C., Hernalsteens, J.P. et al. Transfer of Ti plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4. Molec. Gen. Genet. 152, 119–124 (1977). https://doi.org/10.1007/BF00268807
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00268807