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The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis

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Summary

DNA preparations of the chloramphenicol resistance determining S. aureas plasmids pC194, pC223, and PUB112 can be fractionated by gel electrophoresis into various bands. Electromicroscopic investigations of these various molecular species obtained with pC194 indicated that, depending on the preparations, 70 to 80% of the molecules were monomers, while the rest consisted of various classes of concatemeric and/or interlocked multimers. Measurements of the specific transforming activity of the various molecular classes indicated that the monomers had less than one thousandth the activity of the multimeric plasmid DNA. pC194 DNa of high specific transforming activity could also be obtained by ligation of HindIII generated monomers into concatemeric DNA.

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References

  • Bernhard, K., Schrempf, H., Goebel, W.: Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis. J. Bact. 133, 897–903 (1978)

    Google Scholar 

  • Biswal, N., Kleinschmidt, A.K., Spatz, H.C., Trautner, T.A.: Physical properties of the DNA of bacteriophage SP50. Molec. gen. Genet. 100, 39–55 (1967)

    Google Scholar 

  • Dishman, B.: The role of molecular configuration in transfection of B. subtilis with bacteriophage DNA. Ph.D. thesis, University of Texas (1972)

  • Dubnau, D., Cirigliano, C.: Fate of transforming DNA following uptake by competent Bacillus subtilis. J. molec. Biol. 64, 9–29 (1972)

    Google Scholar 

  • Ehrlich, S.D.: Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc. nat. Acad. Sci. (Wash.) 74, 1680–1682 (1977)

    Google Scholar 

  • Ganesan, A.T., Andersen, J.J., Luh, J., Effron, M.: DNA metabolism in Bacillus subtilis and its phage SPP1. In: Microbiology, pp. 319–325. Washington, D.C.: American Society for Microbiology 1976.

    Google Scholar 

  • Gryczan, T.J., Contente, S., Dubnau, D.: Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bact. 134, 318–329 (1978)

    Google Scholar 

  • Gryczan, T.J., Dubnau, D.: Construction and properties of chimeric plasmids in Bacillus subtilis. Proc. nat. Acad. Sci. (Wash.) 75, 1428–1432 (1978)

    Google Scholar 

  • Hirokawa, H.: Transfecting deoxyribonucleic acid of Bacillus bacteriophage ϕ29 that is protease sensitive. Proc. nat. Acad. Sci. (Wash.) 69, 1555–1559 (1972)

    Google Scholar 

  • Hirokawa, H., Trautner, T.A., Lüder, G.: On the dose response in B. subtilis transfection. Molec. gen. Genet. 156, 263–266 (1977)

    Google Scholar 

  • Hudson, B., Vinograd, J.: Catenated circular DNA molecules in HeLa cell mitochondria. Nature (Lond.) 216, 647–652 (1967)

    Google Scholar 

  • Jaenisch, R., Hofschneider, P.H., Preuss, A.: Isolation of circular DNA by zonal centrifugation. Separation of normal length double length and catenated M13 replicative form DNA and of host specific “episomal” DNA. Biochim. biophys. Acta (Amst.) 190, 88–100 (1969)

    Google Scholar 

  • Kleinschmidt, A., Zahn, R.K.: Über Desoxyribonukleinsäure-Moleküle in Protein-Mischfilmen. Z. Naturforsch. 14b, 770–779 (1959)

    Google Scholar 

  • Kupersztoch, Y.M., Helinski, D.R.: A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli. Biochem. biophys. Res. Commun. 54, 1451–1459 (1973)

    Google Scholar 

  • Löfdahl, S., Sjöström, J.E., Philipson, L.: Characterization of small plasmids from Staphylococcus aureus. Gene 3, 149–159 (1978)

    Google Scholar 

  • Mazza, G., Fortunato, A., Ferrari, E., Canosi, U., Falaschi, A., Polsinelli, M.: Genetic and enzymic studies on the recombination process in Bacillus subtilis. Molec. gen. Genet. 136, 9–30 (1975)

    Google Scholar 

  • Meinke, W., Goldstein, A.: Studies on the structure and formation of polyoma DNA replicative intermediates. J. molec. Biol. 61, 543–563 (1971)b

    Google Scholar 

  • Morris, C.F., Sinha, N.K., Alberts, B.M.: Reconstruction of bacteriophage T4 DNA replication apparatus from purified components: Rollong circle replication following de novo chain initiation on a single-stranded circular DNA template. Proc. nat. Acad. Sci. (Wash.) 72, 4800–4804 (1975)

    Google Scholar 

  • Novick, R.P., Smith, K., Sheehy, R.J., Murphy, E.: A catenated intermediate in plasmid replication. Biochem. biophys. Res. Commun. 54, 1460–1469 (1973)

    Google Scholar 

  • Rottländer, E., Trautner, T.A.: Genetic and transfection studies with B. subtilis phage SP50. I. Phage mutants with restricted growth on B. subtilis strain 168. Molec. gen. Genet. 108, 47–60 (1970)

    Google Scholar 

  • Rutberg, L., Hoch, J.A., Spizizen, J.: Mechanism of transfection with deoxyribonucleic acid from the temperate Bacillus bacteriophage ϕ105. J. Virol. 4, 50–57 (1969)

    Google Scholar 

  • Rutberg, L., Rutberg, B.: Characterization of infectious deoxyribonucleic acid from temperate Bacillus subtilis bacteriophage ϕ105. J. Virol. 5, 604–608 (1970)

    Google Scholar 

  • Schlager, E.J., Spatz, H.Ch.: Specific recognition in gene conversion. The extent of repair synthesis in SPP1 transfection of B. subtilis. Molec. gen. Genet. 130, 165–175 (1974)

    Google Scholar 

  • Thuring, R.W.J., Sanders, J.P.M., Borst, P.: A freeze-squeeze method for recovering long DNA from agarose gels. Analyt. Biochem. 66, 213–220 (1975)

    Google Scholar 

  • Trautner, T.A., Spatz, H.Ch.: Transfection in B. subtilis. Curr. Top. Microbiol. Immunol. 62, 61–88 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Abt. Trautner, Max-Planck-Institut für molekulare Genetik, Ihnestraße 63/73, D-1000, Berlin 33

    Umberto Canosi, Giovanna Morelli & Thomas A. Trautner

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  1. Umberto Canosi
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  2. Giovanna Morelli
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  3. Thomas A. Trautner
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Communicated by E. Bautz

On leave Istituto di Genetica Università di Pavia

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Canosi, U., Morelli, G. & Trautner, T.A. The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis . Molec. Gen. Genet. 166, 259–267 (1978). https://doi.org/10.1007/BF00267617

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  • DOI: https://doi.org/10.1007/BF00267617

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