Summary
Intracellular recordings and injections of procion yellow (PY) were made in parvocellular neurons in hypothalamic slices of female guinea pigs. Eighty-five neurons, with an average resting membrane potential of -35 mV, were recorded in the arcuate (ARC) ventromedial (VM), and in the cellpoor zones between the ARC and VM. Eleven of the ARC neurons and four neurons from the cell-poor zone could be driven antidromically by median eminence (ME) stimulation, nine other neurons from the three areas could be driven orthodromically by stria terminalis (ST) stimulation.
Twenty-eight parvocellular neurons were tested with 17 β-estradiol (E2), which was applied in the bathing medium as the free steroid. Eleven neurons (nine ARC and two cell-poor-zone neurons) were hyperpolarized 2 to 24 mV by 10−10 M E2 concentrations. 10−8 M estrone concentration was without effect on three of these cells. Through the intracellular injection of PY, the estrogen-sensitive neurons (N = 11) were identified as small fusiform cells with few dendrites. Spine-like appendages were found on only one of these cells. None of the larger pyramidal-like neurons of these areas responded to the application of E2.
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References
Andersen P, Bie B, Ganes T, Laursen AM (1978) Two mechanisms for effects of GABA on hippocampal pyramidal cells. In: Ryall RW, Kelly JS (eds) Iontophoresis and transmitter mechanisms in the mammalian central nervous system. Elsevier, New York, pp 179–184
Barker JL (1976) Peptides: roles in neuronal excitability. Physiol Rev 56: 435–452
Bodoky M, Réthelyi M (1977) Dendritic arborization and axon trajectory of neurons in the hypothalamic arcuate nucleus of the rat. Exp Brain Res 28: 543–555
Cross BA, Dyball REJ, Dyer RG, Jones CW, Lincoln DW, Morris JF, Pickering BT (1975) Endocrine Neurons. In: Greep RO (ed) Recent progress in hormone research, vol 31. Academic Press, New York, pp 243–294
Dodd J, Kelly JS (1978) Is somatostatin an excitatory transmitter in the hippocampus? Nature 273: 674–675
Dufy B, Dufy-Barbe L (1979) Effects of gonadal steroids on the electrical activity of hypothalamic neurons. In: Vincent JD (ed) CNRS collogue international sur la biologie cellulaire des processes neurosecretoires hypothalamiques. Edition de CNRS, Bordeaux, pp 207–220
Dufy B, Dufy-Barbe L, Vincent JD (1978) Effects of protein synthesis inhibitors on the negative feedback effect of estrogen on LH release. Horm Res 9: 279–291
Dufy B, Partouche Ch, Poulain D, Dufy-Barbe L, Vincent JD (1976) Effects of oestrogen on the electrical activity of identified and unidentified hypothalamic units. Neuroendocrinology 22: 38–47
Harris MC, Sanghera M (1974) Projection of medial basal hypothalamic neurones to the preoptic anterior hypothalamic areas and the paraventricular nucleus in the rat. Brain Res 81: 401–411
Hayward JN (1976) Functional and morphological aspects of hypothalamic neurons. Physiol Rev 56: 435–452
Heimer L, Nauta WJ (1969) The hypothalamic distribution of the stria terminalis in the rat. Brain Res 13: 284–297
Kelly MJ, Dudley C, Moss RL (1975) Identification of estrogen-sensitive neurons in the preoptic-septal area of the normal cyclic female rat. Society of Neuroscience, 5th Annual Meeting
Kelly MJ, Kuhnt U, Wuttke W (1979a) Effects of 17 β-estradiol on hypothalamic parvocellular neurons as revealed by intracellular recordings and staining with procoin yellow. The Endocrine Society, 61st Annual Meeting
Kelly MJ, Kuhnt U, Wuttke W (1979b) Intracellular electrophysiological studies on the parvocellular neurons of the hypothalamus. Fed Proc 38: 2557
Kelly MJ, Kuhnt U, Wuttke W (1979c) Morphological features of physiologically identified hypothalamic neurons as revealed by intracellular marking. Exp Brain Res 34: 107–116
Kelly MJ, Moss RL, Dudley CA (1976) Differential sensitivity of preoptic septal neurons to microelectrophoresed estrogen during the estrous cycle. Brain Res 114: 152–157
Kelly MJ, Moss RL, Dudley CA (1977a) The effects of microelectrophoretically applied estrogen, cortisol, and acetycholine on medial preoptic septal unit activity throughout the estrous cycle of the female rat. Exp Brain Res 30: 53–64
Kelly MJ, Moss RL, Dudley CA, Fawcett CP (1977b) The specificity of the response of preoptic septal area neurons to estrogen: 17 α-estradiol versus 17 β-estradiol and the response of extrahypothalamic neurons. Exp Brain Res 30: 43–52
Krnjević K (1974) Chemical nature of synaptic transmission in vertebrates. Physiol Rev 54: 418–450
McCann SM (1978) Regulation of secretion of follicle-stimulating hormone and luteinzing hormone. In: Greep RO, Astwood EB (eds) The pituitary gland and its neuroendocrine control, Part 2. American Physiological Society, Washington, DC, (Handbook of physiology, Section 7: Endocrinology, vol IV, pp 489–517)
McEwen BS, Luine VN (1979) Specificity, mechanisms, and functional significance of steroid-receptor interactions in the brain and pituitary. In: Vincent JD (ed) CNRS collogue international sur la biologie cellulaire des processes neurosecretoires hypothalamiques. Edition de CNRS, Bordeaux, pp 239–265
Milhouse OE (1973) The organization of the ventromedial hypothalamic nucleus. Brain Res 55: 71–87
Milhouse OE (1978) Cytological observations on the ventromedial hypothalamic nucleus. Cell Tissue Res 191: 473–491
Moss RL (1976) Unit responses in preoptic and arcuate neurons related to anterior pituitary function. In: Martini L, Ganong WF (eds) Frontiers of neuroendocrinology, vol 4. Raven Press, New York, pp 95–128
Pfaff DW (1968) Autoradiographic localization of radioactivity in rat brain after injection of tritiated sex hormones. Science 161: 1355–1356
Pfaff DW, Keiner M (1973) Atlas of estradiol-concentrating cells in the central nervous system of the female rat. J Comp Neurol 151: 121–158
Renaud LP (1979) Tubero-infundibular neurons in the basomedial hypothalamus of the rat: electrophysiological evidence for axon collaterals to hypothalamic and extrahypothalamic areas. Brain Res 105: 59–72
Renaud LP (1977) Influence of medial preoptic-anterior hypothalamic area stimulation on the excitability of mediobasal hypothalamic neurons in the rat. J Physiol (Lond) 264: 541–564
Renaud LP, Blume HW, Kearney RE, MacKenzie BW, Pittman OJ (1978) Physiological anatomy of the hypothalamus. In: Veale WL, Lederis K (eds) Current studies of hypothalamic function, vol 2. Karger, Basel, pp 1–16
Schwartzkroin PA (1975) Characteristics of CAI neurons recorded intracellularly in the hippocampal in vitro slice preparation. Brain Res 85: 423–436
Schwartzkroin PA (1978) Secondary range rhythmic spiking in hippocampal neurons. Brain Res 149: 247–250
Stumpf WE (1968) Estradiol-concentrating neurons: topography in the hypothalamus by drymount autoradiography. Science 162: 1001–1003
Szentágathai J (1964) The Parvicellular neurosecretory system. In: Bargmann W, Schade JP (eds) Lectures on the Diencephalon. Elsevier, Amsterdam, (Progress in brain research, vol 3, pp 135–146)
Vreeburgh JTM, Schretlen PJM, Baum MJ (1975) Specific high-affinity binding of 17 β-estradiol in cytosols from several brain regions and pituitary of intact and castrated adult male rats. Endocrinology 97: 969–977
Wuttke W (1974) Preoptic unit activity and gonadotropin release. Exp Brain Res 19: 205–216
Yagi K, Sawaki Y (1975) Recurrent inhibition and facilitation: demonstration in the tubero-infundibular system and effects of strychnine and picrotoxin. Brain Res 84: 155–159
Yagi K, Sawaki Y (1978) Electrophysiological characteristics of identified tubero-infundibular neurons. Neuroendocrinology 26: 50–64
Yamamoto C (1972) Activation of hippocampal neurons by mossy fiber stimulation in thin brain sections in vitro. Exp Brain Res 14: 423–435
Zigmond RE, McEwen BS (1970) Selective retention of estradiol by cell nuclei in specific brain regions of the ovariectomized rat. J Neurochem 17: 889–899
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Postdoctoral research fellow of the National Institute of Neurological and Communicative Disorders and Stroke
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Kelly, M.J., Kuhnt, U. & Wuttke, W. Hyperpolarization of hypothalamic parvocellular neurons by 17 β-estradiol and their identification through intracellular staining with procion yellow. Exp Brain Res 40, 440–447 (1980). https://doi.org/10.1007/BF00236152
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DOI: https://doi.org/10.1007/BF00236152