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The 5′ end sequences and exon organization in rat regucalcin gene

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Abstract

The 5−flanking region of the gene for a Ca2−-binding protein regucalcin was cloned from a rat genomic library which was constructed in lambda EMBL3 SP6/T7 vector. The genomic library was screened by using the radiolabeled probe with the 5′ region (0.5 kb) of rat regucalcin complementary deoxyribonucleic acid (cDNA). Positive clone had the 5.5 kb fragment which was hybridized with the 5′-probe. This fragment contained three exons (I–III) of the gene coding for a rat regucalcin. The nucleotide sequence of exons completely agreed with that of a rat regucalcin cDNA clone. A supposed translational initiation site existed in the exon 11. Homology analysis showed that a putative transcription start site in the rat regucalcin gene was located at position 26 downstream from a TATA-box. Another upstream element, a CCAAT box-like sequence, was located at −170. Moreover, there were many regulatory elements (Hox, AP-1, AP-2 and AP-4) in the 5′-flanking region of the rat regucalcin gene. The organization of rat regucalcin gene seemed to be about 18 kb in size and consisted of seven exons and six introns.

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Authors and Affiliations

  1. Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan

    Masayoshi Yamaguchi, Reiko Makino & Noriaki Shimokawa

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  1. Masayoshi Yamaguchi
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  2. Reiko Makino
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  3. Noriaki Shimokawa
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Additional information

The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank Nucleotide Sequence Databases with the following accession number D67071, D67069, and D67070.

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Yamaguchi, M., Makino, R. & Shimokawa, N. The 5′ end sequences and exon organization in rat regucalcin gene. Mol Cell Biochem 165, 145–150 (1996). https://doi.org/10.1007/BF00229476

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  • DOI: https://doi.org/10.1007/BF00229476

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