Summary
The genes coding for the Rubisco small subunit (SSU) and for the α-subunit of the Rubisco-binding protein were located to chromosome arms of common wheat. HindIII-digested total DNA from the hexaploid cultivar Chinese Spring and from ditelosomic and nullisomic-tetrasomic lines was probed with these two genes, whose chromosomal location was deduced from the disappearance of or from changes in the relative intensity of the relevant band(s). The Rubisco SSU pattern consisted of 14 bands, containing at least 21 different types of DNA fragments, which were allocated to two homoeologous groups: 15 to the short arm of group 2 chromosomes (4 to 2AS, 7 to 2BS, and 4 to 2DS) and 6 to the long arm of group 5 chromosomes (2 on each of arms 5AL, 5BL, and 5DL). The pattern of the Rubisco-binding protein consisted of three bands, each containing one type of fragment. These fragments were located to be on the short arm of group 2 chromosomes. The restriction fragment length polymorphism (RFLP) patterns of several hexaploid and tetraploid lines were highly conserved, whereas the patterns of several of their diploid progenitors were more variable. The variations found in the polyploid species were mainly confined to the B genome. The patterns of the diploids T. monococcum var. urartu and Ae. squarrosa were similar to those of the A and D genome, respectively, in polyploid wheats. The pattern of T. monococcum var. boeoticum was different from the patterns of the A genome, and the patterns of the diploids Ae. speltoides, Ae. longissima, and Ae. Searsii differed from that of the B genome.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barraclough R, Ellis RJ (1980) Protein synthesis in chloroplasts. IX. Assembly of newly synthesized large subunits into ribulose bisphosphate carboxylase in isolated pea chloroplasts. Biochim Biophys Acta 608:19–31
Blair GE, Ellis RJ (1973) Protein synthesis in chloroplasts. I. Light-driven synthesis of the large subunit of fraction I protein by isolated pea chloroplasts. Biochim Biophys Acta 319:223–234
Bowman CM (1986) Copy numbers of chloroplast and nuclear genomes are proportional in mature mesophyll cells of Triticum and Aegilops species. Planta 167:264–274
Broglie R, Coruzzi G, Lamppa G, Keith B, Chua N-H (1983) Structural analysis of nuclear genes coding for the precursor to the small subunit of wheat ribulose-1,5-bisphosphate carboxylase. Biotechnology 1:55–61
Chao S, Raines CA, Longstaff M, Sharp PJ, Gale MD, Dyer TA (1989) Chromosomal location and copy number in wheat and some of its close relatives of genes for enzymes involved in photosynthesis. Mol Gen Genet 218:423–430
Chua N-H, Schmidt GW (1978) Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci USA 75:6110–6114
Coen DM, Bedbrook JR, Bogorad L, Rich A (1977) Maize chloroplast DNA fragment encoding the large subunit of ribulose bisphosphate carboxylase. Proc Natl Acad Sci USA 74:5487–5491
Coruzzi G, Broglie R, Gashmore AR, Chua N-H (1983) Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose-l,5-bisphosphate carboxylase and the major chlorophyll a-/b-binding thylakoid polypeptide. J Biol Chem 258:1399–1402
Dean C, Leech RM (1982) Genome expression during normal leaf development. 2. Direct correlation between ribulose bisphosphate carboxylase content and nuclear ploidy in a polyploid series of wheat. Plant Physiol 70:1605–1608
Dean C, Elzen P van den, Tamaki S, Dunsmuir P, Bedbrook J (1985) Linkage and homology analysis divides the eight genes for the small subunit of petunia ribulose-l,5-bisphosphate carboxylase into three genes families. Proc Natl Acad Sci USA 82:4964–4968
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1 [4]:19–21
Dobberstein B, Blobel G, Chua N-H (1977) In vitro synthesis and processing of a putative precursor for the small subunit of ribulose-1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 74:1081–1085
Dvorak J (1988) Cytogenetical and molecular inferences about the evolution of wheat. In: Proc 7th Int Wheat Genet Symp Cambridge, pp 187–192
Ellis RJ (1979) The most abundant protein on earth. Trends Biochem Sci 4:241–244
Ellis RJ, Van der Vies SM (1988) The rubisco subunit binding protein. Photosynth Res 16:101–115
Feldman M, Avivi L, Levy AA, Zaccai M, Avivi Y, Millet E (1990) High protein wheat. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 6: Crops II. Springer, Berlin Heidelberg, pp 593–613
Hemmingsen SM, Woolford C, Van der Vies SM, Tilly K, Dennis DT, Georgopoulos CP, Hendrix RW, Ellis RJ (1988) Homologous plant and bacterial proteins chaperone oligomeric protein assembly. Nature 333:330–334
Highfield PE, Ellis RJ (1978) Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase. Nature 271:420–424
Jellings AJ, Leese BM, Leech RM (1983) Location of chromosomal control of ribulose bisphosphate carboxylase amounts in wheat. Mol Gen Genet 192:272–274
Kawashima N, Wildman SG (1970) Fraction I protein. Annu Rev Plant Physiol 21:325–328
Kawashima N, Wildman SG (1972) Studies on fraction I protein. IV. Mode of inheritance of primary structure in relation to whether chloroplast or nuclear DNA contains the code for a chloroplast protein. Biochim Biophys Acta 262:42–49
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning; a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York/NY
McIntosh L, Poulsen C, Bogorad L (1980) Chloroplast gene sequence for the large subunit of ribulose bisphosphate carboxylase of maize. Nature 288:556–560
Miziorko HM, Lorimer GH (1983) Ribulose-1,5-bisphosphate carboxylase/oxygenase. Annu Rev Biochem 52:507–535
Musgrove JE, Johnson RA, Ellis RJ (1987) Dissociation of the ribulose bisphosphate-carboxylase large-subunit binding protein into dissimilar subunits. Eur J Biochem 163:529–534
Nishikawa K (1983) Species relationship of wheat and its putative ancestors as viewed from isozyme variation. In: Proc 6th Int Wheat Genet Symp Kyoto, pp 59–63
Roy H (1989) Rubisco assembly: a model system for studying the mechanism of chaperonin action. The Plant Cell 1:1035–1042
Simmons SR (1987) Growth, development, and physiology. In: Heyne EG (ed) Wheat and wheat improvement, 2nd edn. American Society of Agronomy, Madison/WI, pp 77–113
Smith SM, Bedbrook J, Speir J (1983) Characterization of three cDNA clones encoding different mRNAs for the precursor of the small subunit of wheat ribulose bisphosphate carboxylase. Nucleic Acids Res 11:8719–8734
Author information
Authors and Affiliations
Additional information
Communicated by J. MacKey
Rights and permissions
About this article
Cite this article
Galili, S., Galili, G. & Feldman, M. Chromosomal location of genes for Rubisco small subunit and Rubisco-binding protein in common wheat. Theoret. Appl. Genetics 81, 98–104 (1991). https://doi.org/10.1007/BF00226118
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00226118