Skip to main content
SpringerLink
Log in

Effect of glucocorticoids on arachidonic acid metabolism and prostaglandin secretion by cultures of newborn rat heart cells

  • Invited Paper
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

We described that oxygen deprivation induced in cultures of heart muscle cells, biochemical events similar to those described in ischemic tissue: arachidonic acid liberation, loss of membrane phospholipids and increase in neutral lipids. Since glucocorticoids have been described to inhibit phospholipase activity and to exert beneficial effects during myocardial infarction, we studied in our experimental model the action of dexamethasone on the metabolism of arachidonic acid and on the synthesis of immunoreactive prostaglandins. Our results show that heart muscle cells produce prostaglandin E2 and 6-keto-prostaglandin-F1α. This synthesis, inhibited by dexamethasone (70% inhibition), decreased after oxygen-deprivation (−45%). The effect of oxygen deprivation and dexamethasone (−60%) are not additive. Moreover, steroid treatment failed to counteract the loss of polyunsaturated fatty acids from the phospholipids, the increase in neutral lipids and the liberation of arachidonic acid induced by oxygen deprivation in muscle cells. These results may indicate that the cardiovascular effects of glucosteroids are not the consequence of a direct effect on heart metabolism at cellular level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Genth K, Hofmann M, Schaper W: Ineffectiveness of methylprednisolone to reduce infarct size in experimental coronary occlusion. Basis Res Cardiol 77: 182–187, 1982

    Google Scholar 

  2. Lefer AM, Crossley K, Grigonis G, Lefer DJ: Mechanism of the beneficial effect of dexamethasone on myocardial cell integrity in acute myocardial ischemia. Basic Res Cardiol 75: 328–339, 1980

    Google Scholar 

  3. Smith GT, Geary G, Ruf W, Fore FN, Oyama M, McNamara JJ: Quantitative effect of a singe large dose of methylprednisolone on infarct size in baboons. Cardiovas Res 7: 408–418, 1980

    Google Scholar 

  4. Roberts R, De Mello V, Sobel BE: Deleterious effects of methyl prednisolone in patients with myocardial infarction. Circulation 53 suppl 1: 204–206, 1976

    Google Scholar 

  5. Funder JW, Duval D, Meyer P: Cardiac glucocorticoid receptors: the binding of tritiated dexamethasone in rat and dog heart. Endocrinol 93: 1300–1308, 1973

    Google Scholar 

  6. Seleznew IM, Martynov AV: Permissive effect of glucocorticoids in catecholamine action in the heart: possible mechanisms. J Mol Cell Cardiol 14 suppl 3: 49–58, 1982

    Google Scholar 

  7. Anastasia JV, Mc Carl RL: Effects of cortisol on cultured rat heart cells. Lipase activity, fatty acid oxydation, glycogen metabolism and ATP levels as related to the beating phenomenon. J Cell Biol 57: 109–116, 1973

    Google Scholar 

  8. Axelrod L: Inhibition of prostacylin production mediates permissive effect of glucocorticoids on vascular tone. Lancet 1: 904–906, 1983

    Google Scholar 

  9. Freyss-Beguin M, Van Brussel E, Lechat P: Metabolic and functional effects of oxygen deprivation on cultures of rat cardiac myoblasts and fibroblasts. Biol Cell 41: 51–58, 1981

    Google Scholar 

  10. Katz AM, Messineo FC: Lipid-membrane interactions and the pathogenesis of ischemic damage in the myocardium. Cir Res 48: 1–16, 1981

    Google Scholar 

  11. Homo-Delarche F, Duval D, Papiernik M: Prostaglandin production by phatocytic cells of the mouse thymic reticulum in culture and its modulation by indomethacin and corticosteroids. J Immunol 135: 506–512, 1985

    Google Scholar 

  12. Chien KR, Han A, Sen A, Buja LM, Willerson IT: Accumulation of unesterified arachidonic acid in ischemic canine myocardium. Relationship to a phosphatidylcholine deacylation-reacylation cycle and the depletion of membrane phospholipids. Cir Res 54: 313–322, 1984

    Google Scholar 

  13. Flower RJ, Blackwell GJ: Antiinflammatory steroids induce biosynthesis of a phospholipase AZ inhibitor which prevents prostaglandin generation. Nature 275: 456–458, 1979

    Google Scholar 

  14. Ahumada GG, Sobel BE, Needleman P: Synthesis of prostaglandins by cultured rat heart myocytes and cardiac mesenchymal cells. J Mol Cell Cardiol 12: 685–700, 1980

    Google Scholar 

  15. Needleman P: The synthesis and function of prostaglandins in the heart. Fed Proc 35: 2376–2381, 1976

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Hôpital Necker, Inserm U7, 161 rue de Sèvres, 75015, Paris, France

    E. Van Millanvoye-Brussel, M. Freyss-Beguin & D. Duval

  2. Inserm U25, Hôpital Necker, 161 rue de Sèvres, 75015, Paris, France

    F. Homo-Delarche

Authors
  1. E. Van Millanvoye-Brussel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Freyss-Beguin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Homo-Delarche
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Duval
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Van Millanvoye-Brussel, E., Freyss-Beguin, M., Homo-Delarche, F. et al. Effect of glucocorticoids on arachidonic acid metabolism and prostaglandin secretion by cultures of newborn rat heart cells. Mol Cell Biochem 88, 123–127 (1989). https://doi.org/10.1007/BF00223433

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00223433

Key words

Search

Navigation