Abstract
The glycosyl phosphatidylinositol (GPI)-linked antigen recognized by monoclonal antibody (mAb) MEM-102 is expressed on all peripheral blood lymphocytes, both resting and activated. Its properties are very similar to a previously described activation antigen, Blast-1. The amino acid sequence deduced from the structure of cloned cDNA is identical to that of the Blast-1 antigen except for a single amino acid residue. There are several other minor differences in the nucleotide sequence of the Blast-1 and MEM-102 cDNAs that do not affect the predicted structure of the polypeptide product. The amino acid sequence of the first 15 N-terminal residues of the antigen purified from Raji cells is found in the deduced sequence close to the presumed boundary between the leader peptide and mature polypeptide. Properties of the recombinant product expressed in COS cells are similar to the antigen isolated from peripheral blood mononuclear cells (PBMNCs) or B-and T-cells lines. The antigen purified on immobilized mAb MEM-102 is recognized by all six known CD48 mAbs under western blotting conditions. COS cells transfected with MEM-102 cDNA react with all the CD48 mAbs. It is concluded that mAb MEM-102 is directed against the as yet poorly characterized antigen CD48, which is therefore structurally closely related to Blast-1. Several possibilities are discussed that might account for the apparent discrepancy between the broad pan-leucocyte expression of the MEM-102/CD48 antigen and much more restricted expression of the epitope recognized by the previously described mAb defining the Blast-1 antigen.
Similar content being viewed by others
References
Bažil, V., Štefanová, I., Hilgert, I., Krištofová, H., Vaněk, S., Bukovský, A., and Hořejší, V.: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35: 289–297, 1989
Gorga, J. C., Hořejší, V., Johnson, D. R., Raghupathy, R., and Strominger, J. L.: Purification and characterization of class II histocompatibility antigens from a homozygous human B cell line. J Biol Chem 262: 16087–16094, 1987
Hadam, M.: Cluster report: CD48. In W. Knapp, B. Dörken, W. R. Gilks, E. P. Reiber, R. E. Schmidt, H. Stein, and A. E. G. Kr. von dem Borne (eds.): Leucocyte Typing IV. White Cell Differentiation Antigens, pp. 661–664, Oxford University Press, Oxford, 1989
Hořejší, V., Angelisová, P., Bažil, V., Krištofová, H., Stoyanov, S., Štefanová, I., Hausner, P., Vosecký, M., and Hilgert, I.: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha) 34: 23–34, 1988
Killeen, N., Moessner, R., Arvieux, J., Willis, A., and Williams, A. F.: The MRC OX-45 antigen of rat leucocytes and endothelium is in a subset of the immunoglobulin superfamily with CD2, LFA-3 and carcinoembryonic antigens. EMBO J 7: 3087–3092, 1988
Matsudaira, P.: Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262: 10035–10038, 1987
Matsui, Y., Shibano, K., Kashiwagi, H., Yamakawa-Kobayashi, K., Inoko, H., Staunton, D. E., and Thorley-Lawson, D. A.: Characterization of genomic polymorphism of an activation-associated antigen, Blast-1. Immunogenetics 31: 188–190, 1990
Meuer, S. C., Hussey, R. E., Fabbi, M., Fox, D., Acuto, O., Fitzgerald, K. A., Hodgdon, J. C., Protentis, J.-P., Schlossman, S. F., and Reinherz, E. L.: An alternative pathway of T-cell activation: a functional role for the 50 kd sheep erythrocyte receptor protein. Cell 36: 897–906, 1984
Sanger, F., Nicklen, S., and Coulson, A.: DNA sequencing with chainterminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977
Schnabl, E., Stockinger, H., Majdic, O., Gaugitsch, H., Lindley, I. J. D., Mauer, D., Hajek-Rosenmayr, A., and Knapp, W.: Activated human T lymphocytes express MHC class I heavy chains not associated with ß2-microglobulin. J Exp Med 171: 1431–1442, 1990
Seed, B.: An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2. Nature 329: 840–842, 1987
Seed, B. and Aruffo, A.: Molecular cloning of the human CD2 antigen, the T cell erythrocyte receptor by rapid immunoselection procedure. Proc Natl Acad Sci USA 84: 3365–3369, 1987
Sewell, W. A., Brown, M. H., Dunne, J., Owen, M. J., and Crumpton, M. J.: Molecular cloning of the human T-lymphocyte surface CD2 (T11) antigen. Proc Natl Acad Sci USA 83: 8718–8722, 1986
Staunton, D. E. and Thorley-Lawson, D. A.: Molecular cloning of the lymphocyte activation marker Blast-1. EMBO J 6: 3695–3702, 1987
Staunton, D. E., Fischer, R. C., LeBeau, M. M., Lawrence, J. B., Barton, D. E., Francke, U., Dustin, M., and Thorley-Lawson, D. A.: Blast-1 possesses a glycosyl-phosphatidylinositol (GPI) membrane anchor, is related to LFA-3 and OX-45 and maps to chromosome 1q21–23. J Exp Med 169: 1087–1101, 1989
Štefanová, I., Hilgert, I., Krištofová, H., Brown, R., Low, M. G., and Hořejší, V.: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol 26: 153–161, 1989
Thorley-Lawson, D. A., Schodey, R. T., Bhan, A. K., and Nadler, L. M.: Epstein-Barr virus superinduces a new human B cell differentiation antigen (B-LAST-1) expressed on transformed lymphoblasts. Cell 30: 415–425, 1982
Author information
Authors and Affiliations
Additional information
The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M 37766.
Rights and permissions
About this article
Cite this article
Kořínek, V., Štefanová, I., Angelisová, P. et al. The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1. Immunogenetics 33, 108–112 (1991). https://doi.org/10.1007/BF00210823
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00210823