Abstract
A group of monoclonal antibodies to the staphylococcal enterotoxins B and C1 without any cross-reactivity to the other known staphylococcal enterotoxins A, C2, C3, D and E was developed. The monoclonal antibodies were compared in competition ELISA's with regard to affinity and epitope recognition. Three different groups could be classified: Group 1, consisting of the MAbs B/3–4 and B/3–8, recognizes in identical manner staphylococcal enterotoxin B. Also group 2 (MAb B/3–5) recognizes only staphylococcal enterotoxin B, yet group 1 and 2 do not compete with each other in the competition ELISA and therefore own different paratopes. Group 3 (MAb C1/2–3 and C14–6) reacts exclusively with staphylococcal enterotoxin C1, not even with staphylococcal enterotoxins C2 and C3.
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Kienle, E., Buschmann, H.G. Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays. Med Microbiol Immunol 178, 127–133 (1989). https://doi.org/10.1007/BF00198011
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DOI: https://doi.org/10.1007/BF00198011