Summary
We report the successive application of classical Giemsa banding and chromosomal in situ suppression hybridization in clinical cytogenetics. The use of both techniques within one protocol requires an additional fixation of the chromosome preparations and an improved suppression of the labelled repetitive sequences. The combination of these two cytological techniques allows the high resolution mapping of translocated Y-chromosomal sequences in the chromosome set of an XX-male.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cremer T, Lichter P, Borden J, Ward DC, Manuelidis L (1988) Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes. Hum Genet 80:235–246
Garson JA, Berghe JA van den, Kemshead JT (1987) Novel non-isotopic in situ hybridization detects small (1kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and β-NGF genes. Nucleic Acids Res 15:4761–4770
Jauch A, Daumer C, Lichter P, Murken J, Schroeder-Kurth TM, Cremer T (1990) Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics. Hum Genet 85:145–150
Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet 80:224–234
Magenis RE, Brown MG, Donlon T, Olson SB, Sheehy R, Tomar D (1985) Structural aberrations of the Y chromosome, including the nonfluorescent Y: cytological origin and consequences. In: Sandberg AA (ed) The Y chromosome, part A: Basic characteristics of the Y chromosome. Liss, New York, pp 537–574
Nederlof PM, Flier S van der, Raap AK, Tanke HJ, Ploeg M van der, Kornips F, Geraedts JPM (1989) Detection of chromosome aberrations in interphase tumor nuclei by non radioactive in situ hybridization. Cancer Genet Cytogenet 42:87–98
Pinkel D, Landegent J, Collins C, Fusco J, Segraves R, Lucas J, Gray JW (1988) Fluorescence in situ hybridization with human chromosome specific libraries: detection of trisomie 21 and translocations of chromosome 4. Proc Natl Acad Sci USA 85:9138–9142
Scherthan H, Lutz S, Metzdorf R, Blin N (1989) Interspecies relationship of a chromosome specific DNA. Hereditas 111:183–188
Schweizer D (1981) Counterstain-enhanced chromosome banding. Hum Genet 57:1–14
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klever, M., Grond-Ginsbach, C., Scherthan, H. et al. Chromosomal in situ suppression hybridization after Giemsa banding. Hum Genet 86, 484–486 (1991). https://doi.org/10.1007/BF00194638
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00194638